Stanford University Mass Spectrometry

SUMS Research Applications Symposium

Enabling Technologies, Future Directions
Thursday October 10, 2019

The SUMS Research Applications Symposium is a full day of mass spec-related research, networking, and information on the latest groundbreaking technologies and applications.  The 2019 SUMS-RAS Keynote Speaker, Prof. Garry Nolan, is a pioneer, combining multiple technologies into exciting new enabling methodologies.  As exemplified by the keynote, the symposium theme "Enabling Technologies, Future Directions" celebrates technological innovations and explores future potential.  Come and connect with an enthusiastic community of scientists, and participate in sparking new scientific opportunities and directions.

CALL FOR ABSTRACTS
Share your mass spec-related research at SUMS-RAS 2019!
Submission deadline: Thursday September 12th
Abstract portal now open!

Present a scientific poster, and enter the poster competition. Posters will be displayed all day, and featured in an expanded morning poster session fueled by refreshments. Poster Competition winners will be awarded a $50 gift card at the closing reception! Also, opt in to have your abstract considered for a Lightning Talk during the symposium. Those selected for Lightning Talks will be notified by Wednesday September 18, 2019. Submit your abstract today!

 

SUMS has moved -- find us in Bass Biology 024  https://mass-spec.stanford.edu/sums-move-bbb

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Welcome to the Vincent Coates Foundation Mass Spectrometry Laboratory.

The laboratory is named in honor of a generous gift from Vincent and Stella Coates, given through the Stanford School of Medicine to support the mass spec & proteomics facility as a shared core resource. It is a Bio-X core facility, embodying the Bio-X spirit of interdisciplinary communication and collaboration. It is also partially supported as the Proteomics Shared Resource of the Stanford Cancer Institute. The Dean of Research provides oversight and support to keep the laboratory's expertise and support available to researchers throughout Stanford University & Medical Center.

 

Overview of Services

Beyond making available state-of-the-art, user-friendly facilities and services, the laboratory enables education, methods development, and new applications development, designed to meet the rapidly evolving needs of researchers. Due to the essential information that mass spectrometry provides to researchers in the fields of the physical and life sciences, medicine, and engineering, the laboratory serves as an “intellectual watering hole” at the crossroads of diverse disciplines.

At this time, we have 16 instruments in operation: three GC-MS and twelve LC-MS systems -- four single quads, one ion trap, four triple quads, one hybrid quadrupole-time of flight, one Orbitrap (Exactive), one Q-Exactive, two hybrid LTQ-Orbitraps, and a TIMS-TOF -- as well as a MALDI-TOF. Software resources include instrument-specific packages Xcalibur, MassLynx, ChemStation; proteomics software Byonic, Mascot, Sequest Sorcerer, Scaffold; and other tools LCquan, QuanLynx, OpenLynx, MetaboLynx, ChemStation, Mass Hunter, Spectral Works.

Routine mass spec and proteomic services include molecular weight determination, MSn, LC-MS, high resolution MS, bottom-up proteomics, and more. Please contact SUMS to discuss custom work such as complex proteomic analysis, relative and targeted quantitation, drug discovery support, de novo peptide sequencing, new application development, and other projects.

Types of Service and Analysis Offered by SUMS:

  • Proteomics
    • Protein identification - gel or solution based
    • Shotgun proteomics, e.g. cell lysates, tissue preps
    • Analysis of frozen or FFPE banked tissues
    • Post-translational modification studies, e.g. phosphorylation, methylation, ubiquitination
    • Quantitative studies - labeled and unlabeled differential expression; targeted absolute quantitation
    • Protein characterization - intact mass, peptide mapping
    • Protein interactions - SNAP, crosslinking
  • Small Molecules
    • Molecular weight confirmation
    • High resolution molecular formula confirmation (HRMS)
    • Structural elucidation (MSn)
    • Targeted quantitation
  • Drug Discovery Support
    • Pharmacokinetic (PK) assessment
    • Microsome incubations
    • Metabolite identification
    • Stability assessment
    • Formulation assessment
    • Degradation studies
  • Assays & Quantitation
    • For small molecules and proteins/peptides
    • Pre-established assays for select classes of molecules
    • Custom assay development for molecules of interest
  • Metabolomics
    • Targeted, Quantitative
    • Semi-Quantitative
  • Open access (Stanford Users Only)
    • LC-MS
    • GC-MS
    • MALDI-TOF
  • Other custom projects - contact SUMS to discuss

 

Getting Started

  • Stanford Users:  LOGIN with valid Stanford credentials (SUNet ID).
  • Non-Stanford Users:  LOGIN using iLab credentials. Or, click to SIGN UP for an iLab account.
 

Leadership

Allis Chien, PhD | SUMS Director

Office (Bass 026):  (650) 723-0710

Lab    (Bass 023):  (650) 725-9769

Email:  allis at stanford.edu

 

Financial Contact

William Lee | Accounting Associate

Office    (Bass 024):  (650) 725-9769

Email:  wlee2 at stanford.edu

 

Staff

Name

 

Role

 

Phone

 

Email

 

Location

Ludmila Alexandrova, PhD

 

Drug discovery support, PK, Metabolite ID

 

725-9769

 

ludmilaa at stanford.edu

 

Bass 024

Karolina Krasinska, MS

 

Quantitative MS

 

725-9769

 

krasin at stanford.edu

 

Bass 024

Ryan Leib, PhD

 

Director of Proteomics

 

725-9769

 

rdleib at stanford.edu

 

Bass 024

Fang Liu, PhD

 

Proteomics

 

725-9769

 

fliu7 at stanford.edu

 

Bass 024

Rowan Matney, BA

 

Proteomics

 

725-9769

 

rmatney at stanford.edu

 

Bass 024

Theresa McLaughlin, MS

 

Lab Manager, HRMS, Open access lab, Custom projects

 

725-9769

 

tmcl at stanford.edu

 

Bass 024

Kratika Singhal, MS

 

Proteomics

 

725-9769

 

ksingh29 at stanford.edu

 

Bass 024

 

 

Location and Hours of Operation 

Location

Hours

SUMS, 327 Campus Drive

Room: 024

Stanford University, Stanford, CA 94305-4401

Phone: (650) 725-9769

Fax:  (650) 725-0259

Email:  spectrometry@stanford.edu

9 a.m. - 5 p.m., Monday to Friday  

24/7 access for trained Open Access users

 

 

 

Click to view SUMS location on Stanford Campus Map

 

Links and Resources

    

Publication Acknowledgement 

In accordance with Dean's office policy: If work done with SUMS produces data in a publication, you must acknowledge us ("Vincent Coates Foundation Mass Spectrometry Laboratory, Stanford University Mass Spectrometry") in the publication. Further, if SUMS staff provide significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the PI), then it is expected that these individuals will be coauthors on the publication.  

 

In addition, please acknowledge the following:

NIH requirement for Orbi data: Each publication, press release or other document that uses data from the LTQ-Orbitrap Velos instrument must include an acknowledgement of NIH grant support and disclaimer such as the following:  "The project described was supported by Award Number S10RR027425 from the National Center for Research Resources. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Center for Research Resources or the National Institutes of Health."

 

Stanford Cancer Institute acknowledgement:  As a Stanford Cancer Institute Shared Resource, our lab receives grant support from the National Cancer Institute, which should be acknowledged in all publications. The following statement is suggested: This work was supported in part by NIH P30 CA124435 utilizing the Stanford Cancer Institute Proteomics/Mass Spectrometry Shared Resource.

 

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Contacts

Name Role Phone Email Location
Allis Chien, Ph.D.
Director
 
650.723.0710
 
allis at stanford.edu
 
Bass 026
 
Theresa McLaughlin, MS
Lab Manager
 
650.725.9769
 
tmcl at stanford.edu
 
Bass 024
 
William Lee
Finance
 
650.725.9769
 
wlee2 at stanford.edu
 
Bass 024