Stanford University Mass Spectrometry

 Welcome to the Vincent Coates Foundation Mass Spectrometry Laboratory.

 

The laboratory is named in honor of a generous gift from Vincent and Stella Coates, given through the Stanford School of Medicine to support the mass spec & proteomics facility as a shared core resource. It is a Bio-X core facility, embodying the Bio-X spirit of interdisciplinary communication and collaboration. It is also partially supported as the Proteomics Shared Resource of the Stanford Cancer Institute. The Dean of Research provides oversight and support to keep the laboratory's expertise and support available to researchers throughout Stanford University & Medical Center.

 

  Oct 26, 2017

Keynote speaker: Prof. Alice Ting
Save the date!



Overview of Services

Beyond making available state-of-the-art, user-friendly facilities and services, the laboratory enables education, methods development, and new applications development, designed to meet the rapidly evolving needs of researchers. Due to the essential information that mass spectrometry provides to researchers in the fields of the physical and life sciences, medicine, and engineering, the laboratory serves as an “intellectual watering hole” at the crossroads of diverse disciplines.

At this time, we have in operation three GC-MS and ten LC-MS systems -- four single quads, one ion trap, four triple quads, one hybrid quadrupole-time of flight, one benchtop Orbitrap and two hybrid LTQ-Orbitraps -- as well as a MALDI-TOF. Software resources include instrument-specific packages Xcalibur, MassLynx, ChemStation; proteomics software Byonic, Mascot, Bioworks, Sequest Sorcerer, Scaffold; and other tools Mass Frontier, QuanLynx, OpenLynx, MetaboLynx, ChemDraw.

Routine mass spec and proteomic services include molecular weight determination, MSn, LC-MS, high resolution MS, bottom-up proteomics, and more. Please contact SUMS to discuss custom work such as complex proteomic analysis, relative and targeted quantitation, drug discovery support, metabolomics, de novo peptide sequencing, new application development, and other projects.

Types of Service and Analysis Offered by SUMS:

  • Proteomics
    • Protein identification - gel or solution based
    • Shotgun proteomics, e.g. cell lysates, tissue preps
    • Analysis of frozen or FFPE banked tissues
    • Post-translational modification studies, e.g. phosphorylation, methylation, ubiquitination
    • Quantitative studies - labeled and unlabeled differential expression; targeted absolute quantitation
    • Protein characterization - intact mass, peptide mapping
  • Small Molecules
    • Molecular weight confirmation
    • High resolution molecular formula confirmation (HRMS)
    • Structural elucidation (MSn)
    • Quantitation
  • Drug Discovery Support
    • Pharmacokinetic (PK) assessment
    • Microsome incubations
    • Metabolite identification
    • Stability assessment
    • Formulation assessment
    • Degradation studies
  • Assays & Quantitation
    • For small molecules and proteins/peptides
    • Pre-established assays for select classes of molecules
    • Custom assay development for molecules of interest
  • Metabolomics
    • Targeted, Quantitative
    • Semi-Quantitative
  • Open access (Stanford Users Only)
    • LC-MS
    • GC-MS
    • MALDI-TOF
  • Other custom projects - contact SUMS to discuss

 

Getting Started

  • Stanford Users:  LOGIN to access using valid Stanford credentials (SUNet ID).
 

Leadership

Allis Chien, PhD | SUMS Director

Office (Mudd 167A):  (650) 723-0710

Lab    (Mudd 175):  (650) 725-9769

Email:  allis at stanford.edu

 

Staff

Name

 

Role

 

Phone

 

Email

 

Location

 

 

 

 

 

 

 

 

 

Chris Adams, PhD

 

Director of Proteomics

 

725-9769

 

adamscm at stanford.edu

 

Mudd 167A

Ludmila Alexandrova, PhD

 

Drug discovery support, PK, Metabolite ID

 

725-9769

 

ludmilaa at stanford.edu  

 

Mudd 167B

Karolina Krasinska, MS

 

Quantitative MS

 

725-9769

 

krasin at stanford.edu

 

Mudd 167B

Ryan Leib, PhD

 

Bioinformatics

 

725-9769

 

rdleib at stanford.edu

 

Mudd 167B

Theresa McLaughlin, MS

 

Lab Manager, HRMS, Open access lab, Custom projects

 

725-9769

 

tmcl at stanford.edu

 

Mudd 167A

Roasa Mehmood, BS

 

Proteomics

 

725-9769

 

roasam at stanford.edu

 

Mudd 167B

Kratika Singhal, MS

 

Proteomics

 

725-9769

 

ksingh29 at stanford.edu

 

Mudd 167B

Yuxi (Rachel) Wu, BS

 

Finance

 

725-9769

 

yuxiwu at stanford.edu

 

Mudd 167B

 

Lab Email:  spectrometry@stanford.edu

 

Location and Hours of Operation 

Location

Hours

SUMS, 333 Campus Drive, MUDD 175, MC 5080

Stanford University, Stanford, CA 94305-4401

Phone: (650) 725-9769

Fax:  (650) 725-0259

Email:  spectrometry@stanford.edu

9 a.m. - 5 p.m., Monday to Friday  

24/7 access for trained Open Access users

 

 

 

Click to view SUMS location on Stanford Campus Map

 

Links and Resources

    

Publication Acknowledgement 

In accordance with Dean's office policy: If work done with SUMS produces data in a publication, you must acknowledge us ("Vincent Coates Foundation Mass Spectrometry Laboratory, Stanford University Mass Spectrometry") in the publication. Further, if SUMS staff provide significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the PI), then it is expected that these individuals will be coauthors on the publication.  

 

In addition, please acknowledge the following:

NIH requirement for Orbi data: Each publication, press release or other document that uses data from the LTQ-Orbitrap Velos instrument must include an acknowledgement of NIH grant support and disclaimer such as the following:  "The project described was supported by Award Number S10RR027425 from the National Center for Research Resources. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Center for Research Resources or the National Institutes of Health."

 

Stanford Cancer Institute acknowledgement:  As a Stanford Cancer Institute Shared Resource, our lab receives grant support from the National Cancer Institute, which should be acknowledged in all publications. The following statement is suggested: This work was supported in part by NIH P30 CA124435 utilizing the Stanford Cancer Institute Proteomics/Mass Spectrometry Shared Resource.

 

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Contacts

Name Role Phone Email Location
Allis Chien, Ph.D.
Director
 
650.723.0710
 
allis at stanford.edu
 
Mudd 167A
 
Theresa McLaughlin, MS
Lab Manager
 
650.725.9769
 
tmcl at stanford.edu
 
Mudd 167B
 
Yuxi (Rachel) Wu
Finance
 
650.725.9769
 
yuxiwu at stanford.edu
 
Mudd 167B