Transgenic, Knockout, and Tumor Model Center

 

          

Overview of Services

 

 Welcome to Transgenic, Knockout, and Tumor Model Center

 

Partly supported by funds from Stanford Cancer Institute, the Stanford Transgenic, Knockout, and Tumor Model Center is set to produce your transgenic, knockout and cancer mouse models. The facility provides low-cost DNA microinjection services, ES cell manipulation, microinjection and tumor modeling services to all Stanford as well as external investigators. The facility also provides services on timed pregnant females, strain rederivation, embryo and sperm cryopreservation and IVF procedures for resurrecting frozen sperm.  The Center can also provide transgenic animal tumor models and a variety of dosing/surgical services for in vitro and in vivo tumor studies.

 

Dr. Vittorio Sebastiano is the Director of the facility with responsibility for its daily operation.  Dr. Barry Behr is the Faculty Advisor who oversees the strategic direction of the facility. The facility has an advisory board consisting of Dr. Julien Sage (Pediatrics), Dr. Sherri Green (Comparative Medicine), and Dr. Dean Felsher (Oncology). Oversight is also provided by the Cancer Institute senior leadership to ensure that the needs of Cancer Institute members are met. Dr. Hong Zeng is senior Research Scientist,  Dr. Jeewon Kim is a Research Scientist; Yanfeng Li, Jennifer Lin, Jieying Yang and Shelly Xiao are research staffs of the facility. 

 

For tumor model projects, the client should provide clear written instructions to the staff for proper execution of the project. TKTC will expend all reasonable efforts to follow the written instructions, but cannot guarantee the outcome of an experiment. If the instructions were followed properly, then any repeat experiments are the financial responsibility of the investigator. The repeat of a failed experiment by TKTC at it’s expense requires the prior approval of the director of TKTC.

If the investigators do not have pre-approved animal protocols, they are encouraged to contact us 1.5-2months before the expected start date of the experiment. TKTC will charge fees for writing it up or revising the protocol and getting it approved. 

 

 

Getting Started

  

  • Stanford Users:  LOGIN or REGISTER for an iLab account using valid Stanford credentials (SUNet ID).

 

 

 

  

What's New

 

 

 

 

Eligibility

 

All Stanford investigators who have approved A-PLAC (Administrative Panel on Laboratory Animal Care) protocol numbers are eligible to use the facility's services. The facility has an approved protocol #12002 which covers the production of transgenic and knockout mice and protocol #19267 which covers the production of tumor-bearing mice. Investigators only need protocols for characterization of the transgenic and chimeric animals. It usually takes 4-6 weeks to get a protocol approved by A-PLAC; so you should plan ahead and have a protocol number ready when requesting the service.

 

External users who have an approved animal protocol and animal housing location are also eligible to use the services provided by the TKTC.

 

If you have questions regarding animal protocols, you may contact Dr.Claude Nagamine at 498-4773 or Valerie Fratus at 723-4550.

 

Pricing for External Users

 

Users not paying with an internal Stanford PTA will have to pay a Facilities and Administration (F&A) charge of 58% (starting 1 Sept. 2015).  Exceptions will be made for payments from Stanford Affiliates (organizations with a formal affiliation agreement with Stanford) and for payments from U.S. accredited, degree-granting institutions of higher education using their own institution’s non-sponsored funding (who will pay 8% F&A).

 

Leadership

 

Vittorio Sebastiano, Ph.D. | TKTC Director

Office:  (650)

Email:  vsebast at stanford.edu

 

 

Location and Hours of Operation

 

Location

Hours

Transgenic, Knockout, and Tumor Model Center

Stanford University Medical Center

Room 0137, CCSR Building

Stanford, CA 94305

Tel:  (650) 725-6871

9 a.m. - 5 p.m., Monday to Friday  

 

 

 

 

 

Links and Resources

 

    

 

Publication Acknowledgment

 

As with all Stanford Service Centers, credit must be given to Stanford Transgenic, Knockout, and Tumor Model Center (TKTC) for data that results in a publication. If the work done at Stanford TKTC produces data resulting in a figure in a publication, you are required to acknowledge Stanford TKTC in the publication. Further, if Stanford TKTC staff members provided significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the PI), then it is expected that these individuals will be coauthors on the publication.

 

Contacts

Name Role Phone Email Location
Vittorio Sebastiano, Ph.D.
Director
 

 
vsebast at stanford.edu
 
SIM1 G3078A
 
Hong Zeng, Ph.D.
Sr. Scientist and manager
 
650-724-9556
 
hongzeng at stanford.edu
 
CCSR#0137A
 
Jeewon Kim, Ph.D.
Research Scientist
 
650-721-1485
 
jwonkim at stanford.edu
 
CCSR 0137
 
Primary Contact - TKTC

 
(650) 725-6871
 

 
CCSR Room 0137