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Animal Histology Services (AHS)

Please do not submit orders for AHS via iLab.

AHS is located at the Comparative Medicine Pavillion (CMP), Room SR125.

 

**Submitting Samples**  

Sample drop-off/pick-up cart: Edwards Building (07-309) Room R322 (3rd floor).

Pick-up and drop-off days are daily for internal AHS submissions. 

NOTE: If submitting tissue cassettes, you must submit them in 70% ethanol in a tight-sealing, leak-proof container.

 

All requests must be accompanied by a completed AHS Request form.

 

If you have questions about avaiable services or sample submission (including request forms and price sheets) please contact us at animalhistology@lists.stanford.edu

 

Overview of Services

 

 

Comparative Medicine's Animal Histology Services (AHS) provides a comprehensive range of histological research techniques that include:

  • Standard tissue processing and paraffin embedding
    • Standard and oversized specimens
  • Special orientation of tissues
    • Including orientation to match images from MRI, CT, and other modalities
    • Special consultation required
  • Serial sectioning, step sectioning, and sectioning of frozen specimens

 

In addition to routine hematoxylin and eosin staining (H&E) of tissue sections, we offer a variety of special stains to highlight specific cell types, cellular elements, tissue components, or infectious organisms. Additionally, we offer immunohistochemical / immunoperoxidase and apoptosis assays.

 

Details of services provided through AHS

 

Tissue processing ONLY

  • Tissue processing encompasses the preparation of previously fixed tissues for paraffin embedding. 
  • Tissue processing involves a series of dehydration, clearing, and paraffin (wax) embedding steps.
  • We offer a "processing only" service, which allows investigators to embed the processed tissues themselves so they can spatially orient the specimens in a way that best suits their specific experimental needs.

 

Tissue processing AND embedding

  • After the tissues are processed, they can be embedded in paraffin to make paraffin tissue blocks, that can then be sectioned with a microtome. 
  • We offer tissue processing and paraffin embedding services with the final result being a paraffin tissue block.
  • Additionally, we offer a variety of sectioning and staining services including: 1) the production of unstained slides, 2) routine H&E staining, 3) histochemical ("special") stains, and 4) immunohistochemistry / immunoperoxidase.  Please see the following sections for a brief explanation of these services.  

 

H&E staining

  • Hematoxylin and eosin (H&E) staining is one of the principal and most widely used stains in histology and is generally regarded as the "gold standard" for evaluation of bright field histology specimens. 
  • Additionally, evaluation of H&E preparations is necessary for providing a context in which to adequately interpret observations made in other specialized staining techniques such as histochemical ("special") and immunohistochemical stains. 
  • We provide H&E staining for the tissues and slides we process, or as a stand-alone service (H&E only) on unstained slides of either paraffin-embedded or frozen sections. 

 

Re-cuts with or without staining

  • Once paraffin tissue blocks are made (see "Tissue processing and embedding" above), they can be sectioned with a microtome multiple times for a variety of purposes.
  • Some of the uses for re-cuts include: 1) production of unstained slides for staining at a later time, 2) routine H&E staining, and/or 3) other advanced staining techniques (histochemical "special" stains and/or immunohistochemistry).
  • We offer microtome sectioning services ("re-cuts") of paraffin blocks with or without additional staining, as well as serial and step sectioning. 

 

Histochemical ("special") stains

  • Histochemical ("special") stains aid in the visualization and/or identification of structures and/or substances in tissue sections. 
  • Different special stains can be used to identify cellular components (e.g. intra-cellular lipid, intercalated discs in cardiac cells), cellular accumulations (e.g. iron, copper), matrical and connective tissue elements (e.g. collagen, elastin), and infectious elements (e.g. fungi, bacteria, protozoa). 
  • Please consult with us for the most appropriate stain(s) for your experimental needs. 
  • We strongly recommend always doing a paired H&E-stained section (gold standard) to provide a context in which to interpret observations made in histochemical preparations. 

 

Immunohistochemistry 

  • Immunohistochemistry is based on the principle of antibody binding, and is used to identify specific antigens and specific antigenic determinants (epitopes) in tissue sections.
  • Immunohistochemistry allows for identification and differentiation of specific cell types (e.g. smooth muscle cells, T-lymphocytes, endothelial cells), cellular components (e.g. mitochondria), and specific matrical and connective tissue elements (e.g. collagen IV). 
  • We offer antibody optimization and antibody staining services. 
  • We strongly recommend always doing a paired H&E-stained section (gold standard) to provide a context in which to interpret observations made in immunohistochemical preparations

 

Getting Started

Publication Acknowledgement

As with all Stanford Service Centers, credit must be given to the Comparative Medicine Animal Histology Service Center for data that results in a publication. If the work done at the Comparative Medicine Animal Histology Service Center produces data resulting in a figure in a publication, you are required to acknowledge the Comparative Medicine Animal Histology Service Center in the publication. Further, if the Comparative Medicine Animal Histology Service Center staff members provided significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the PI), then it is expected that these individuals will be coauthors on the publication.

 

Leadership

 

Eric Peterson
Histotechnologist
Manager, AHS
Phone: 650-497-3901
Email: ericpete@stanford.edu

 

Dr. José G. Vilches-Moure
Veterinary Pathologist
Director, AHS
Phone: 650-723-8680
Email: jvilches@stanford.edu

 

Michael John Renzi
Director of Finance & Administration
Financial
Phone: 650-725-3874
Email: mjrenzi@stanford.edu

 

Anne Lum
Assistant Director of Finance and Administration
Financial
Phone: 650-498-5254
Email: annelum@stanford.edu

 

Core Staff

 Eric Peterson  ericpete@stanford.edu  650.497.3901    
         


Location and hours of operation

Hours Location

 

8 am to 5 pm

 

1230 Welch Road

CMP SR125
Stanford, CA, 94305

*The hours of operation are 8:00am-5:00pm, Monday through Friday (unless otherwise specified). 

Links and Resources

 

Contacts

Name Role Phone Email Location
Michael John Renzi
DFA
 
650-724-3874
 
mjrenzi@stanford.edu
 
Edwards R321
 

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