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Cryo-EM Facility

Overview of Services

 

Welcome to the iLabs Reservation Site for the Stanford CEMC (cryo-EM center)! We are a service center focused on cryo-Transmission Electron Microscopy (cryo-TEM) located in the School of Medicine servicing all Stanford Researchers, no matter your skill level, department, or School. We are here to support both single particle (SPA) and tomography projects.

Our resources include:

1 Aquilos2 cryoFIB-SEM with cryoCLEM capabilities (Fairchild)

1 Titan Krios G2 300 KV microscope with Gatan K3 camera and Bioquantum energy filter (TEM1) (SLAC)

1 Titan Krios G2 300 KV microscope with Falcon 4i camera and Selectris energy filter (TEM4) (SLAC)

2 Glacios 200 KV microscopes, one with a K3 and one with Falcon 4i (Fairchild and ChemH, respectively)

1 Morgagni 100 KV microscope for negative staining (Fairchild)

2 Vitrobot plunge freezers (Fairchild and ChemH)

2 Leica GP plunge freezers (Fairchild)

Clipping stations, negative stain bench, and glow dischargers (Fairchild and ChemH)

 

Note: For resources related to ChemH, please contact Haoqing Wang (hwangab@stanford.edu )

 

Our services include:

Training available to New Users (required to use equipment)

Supported sessions available to Basic Users (you do the work, we support)

No support available to Super Users (you are an expert and can operate the microscopes independently)

Full-service support available to any user (we do all the work from grid to data collection)

Collaboration available to any user (we consult and contribute intelligence to the project)

Limited consumables purchase available to any user (grids, c-clips, rings, and cryo-boxes)

 

To begin using equipment at any time: 1. Register for iLabs. 2. Submit a “Request Services” form on the tab and follow-up with an email. You can contact the staff or manager to discuss the feasibility of a project.

 

USER Definition and guidance:

  • New Users must be trained by facility staff before becoming eligible to book as a “basic user”.
  • Basic Users are limited to day time slots. Overnight slots/Monday slots are available upon request, but must be coordinated with facility staff. Potential hourly staff rates may be incurred. 
  • Super Users may reserve day time, evening or weekend/Monday slots. Super users must be certified by the Service Center Operations Director

  

Leadership

Professor Axel Brunger PhD, Executive Director Interim

William Arnold, PhD, Cryo-EM Scientific Manager

 

Staff

Bharti Singal PhD, CryoEM Staff Scientist (bsingal at stanford dot edu)

Haoqing Wang PhD, CryoEM Staff Scientist

David Fernandez-Martinez PhD, CryoEM Staff Scientist

Christopher Haley PhD, Computational Resources and Data Management Specialist

 

Location and hours of operation

 

Hours Location

Staff Hours: Mon-Fri 9am-5pm

Self-Op Availability: 24/ 7                     

Cryo-EM Facility
299 Campus Drive West
Fairchild Building D045

Publication Acknowledgement

As per the Dean's office policy, if data from your work with cEMC (Cryo-electron Microscopy Center) ends up in a publication, it's essential to acknowledge "Stanford University Cryo-electron Microscopy Center (cEMC)" in that publication. Additionally, if the cEMC staff significantly contribute to experimental design, data interpretation, or provide intellectual input, as determined by the guidelines evaluated by the PI, they should be listed as coauthors of the publication. 

Links and Resources

https://med.stanford.edu/cemc

 

 

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