Welcome!
ALERT Due to the COVID-19 pandemic, nearly all Stanford scientific core facilities using the iLab system have ceased lab operations. Exceptions are the TKTC, which is offering mouse sperm and embryo cryopreservation services and the Fly Facility, which continues to make Drosophila food. Online services, such as the Genetics Bioinformatics Service Center and new on-line data analysis options at SCi3, continue to be available. The staffs of all of the core facilities remain available (on-line, not in-person) for discussions including experiment planning, data analysis, and manuscript preparation.
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Welcome to the Cell Sciences Imaging Facility at the Shriram Center
The Cell Sciences Imaging Facility (CSIF) is a University service center that provides high resolution, state-of-the-art technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials. The CSIF operates two sites at Stanford University: The CSIF at SOE Shriram center and the CSIF at SOM Beckman cent. Both facility sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval). You are on CSIF's Shriram iLabs Solutions site.
The CSIF at Shriram provides access to and training on advanced fluorescence and transmitted light microscopy including: laser scanning spectral confocal with AiryScan, multi-photon imaging (Zeiss LSM780, AiryScan, DeepSee), spinning disk confocal microscope with TIRF and photo-activation capabilities (Nikon SDC), FLIM and widefield (epi-fluorescence, polarized light and darkfield, Zeiss AxioImager) microscopes. Detailed information on these microscopes can be found in the Schedule Equipment tab above. Quick links to the microscopes scheduling calenders are below.
The CSIF at Beckman houses both light and electron microscopy services. Light microscopy systems: Upright and inverted confocal laser scanning systems (Zeiss LSM880, AiryScan, AiryScan FAST; Leica SP8 with resonant), upright LEICA SP5 (with long working distance immersion lens) +FLIM and OMX SIM (STORM, TIRF) are accessible by users. FESEM and TEM are available with our full service EM facility. Click this link to access CSIF Beckman Center iLab site.
Both facilities house data processing software for 3D volume rendering and image analysis (Bitplane Imaris, PerkinElmer Volocity, SVI Huygens Deconvolution, ImageJ FIJI). Training, as well as assistance with experimental design, is available on all key technologies.
The CSIF is a member of and is supported by: The SOM Beckman Center, Stanford Cancer Institute, BioX and the SOE
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CSIF Director | Jon Mulholland
Office (SOM Beckman Center, B050A): (650) 725-7532
Email: jwm at stanford.edu
Shriram Center Faculty Advisor | Dr. Alex Dunn
| Name | Role | Phone (650) | Location | |
| Cedric Espenel | CSIF at Shriram manager, advanced imaging specialist | 498-3559 | espenel at stanford.edu | Shriram B023 |
| Marcin Walkiewicz | BioAFM Specialist, SPM Lab Manager | 353-6307 | marcin.walkiewicz at stanford.edu | McCullough 236 |
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Location |
Hours |
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Shriram Center for Bioengineering & Chemical Engineering 443 Via Ortega B023 Cell Sciences Imaging Facility Stanford, CA 94305 |
8 a.m. - 5 p.m., Monday to Friday 24/7 access for trained users
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As with all Stanford Service Centers, credit must be given to Cell Sciences Imaging Facility for data that results in a publication. If the work done at Cell Sciences Imaging Facility produces data resulting in a figure in a publication, you are required to acknowledge Cell Sciences Imaging Facility in the publication. Further, if Cell Sciences Imaging Facility staff members provided significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the PI), then it is expected that these individuals will be coauthors on the publication.
Additionally, almost all of the CSIF's microscopes were obtained via NIH instrumentation grants and therefore require proper acknowmedgement. Please see the required NIH citation information included on the microscope's description page or contact the CSIF director.
| Name | Role | Phone | Location | |
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| Jon Mulholland |
Director of CSIF
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650-725-7532
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jwm@stanford.edu
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Beckman B050A
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| Cedric Espenel |
CSIF Shriram Manager
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650-498-7409
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espenel@stanford.edu
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Shriram B023
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| Marcin Walkiewicz |
Bio AFM, SPM Lab Manager
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650-353-6307
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marcin.walkiewicz@stanford.edu
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McCullough 236
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| Price List |
| ► Equipment / Resources (4) | |||
| Name | Description | Price | |
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| Axioimager Epifluorescence Widefield | Inquire | ||
| LSM780 Confocal/Multiphoton | (view additional details) | Inquire | |
| Nikon Spinning Disk Confocal | (view additional details) | Inquire | |
| Work Station - Imaris | (view additional details) | Inquire | |
| ► Services and Training (3) | |||
| Name | Description | Price | |
| Bruker Resolve Bio-AFM training | (view additional details) | Inquire | |
| Microscope Training & Usage Set-Up | (view additional details) | Inquire | |
| Technical Assistance / Consulting (per hour) | (view additional details) | Inquire | |
| ► Supplies (8) | |||
| Name | Description | Price | |
| Bioptech chamber/Glass-bottom 40mm Willco dish | Inquire | ||
| Fluoro Dish 23mm (small) | Inquire | ||
| Fluoro Dish 40mm (large) | Inquire | ||
| High performance #1.5 cover glass (100/box) | (view additional details) | Inquire | |
| Materials | Inquire | ||
| Mattek Chamber | Inquire | ||
| Nunc Chamber | Inquire | ||
| POC-R coverslip | Inquire | ||
| ► Equipment / Resources (5) | ||||||||||||||||||
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