Welcome!
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Welcome to the Cell Sciences Imaging Facility at the Shriram Center
The Cell Sciences Imaging Facility (CSIF) is a University service center that provides high resolution, state-of-the-art technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials. The CSIF operates two sites at Stanford University: The SOE Shriram Center CSIF and the SOM Beckman Center CSIF. Both facility sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval). You are on the Shriram Center CSIF iLabs Solutions site.
The Shriram Center CSIF provides access to and training on advanced fluorescence and transmitted light microscopy including: laser scanning spectral confocal with AiryScan, multi-photon imaging (Zeiss LSM780, AiryScan, DeepSee), spinning disk confocal microscope with TIRF and photo-activation capabilities (Nikon SDC), FLIM and widefield (epi-fluorescence, polarized light and darkfield, Zeiss AxioImager) microscopes. Detailed information on these microscopes can be found in the Schedule Equipment tab above. Quick links to the microscopes scheduling calenders are below.
The Beckman Center CSIF houses both advanced fluorescence microscopy and electron microscopy instrumentation. Advanced microscope technologies include super resolution (SIM, gSTED, PALM/STORM), laser scanning confocal, multi-photon, FLIM, TIRF and widefield deconvolution as well as scanning and transmission electron microscopy. The Beckman Center CSIF iLabs site is here.
Both facilities offer advanced software for 3D volume rendering and image analysis (Bitplane Imaris, PerkinElmer Volocity, SVI Huygens Deconvolution, ImageJ FIJI). Training, as well as assistance with experimental design, is available on all key technologies.
The CSIF is a member of and support by: The SOM Beckman Center, Stanford Cancer Institute, BioX and the SOE
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CSIF Director | Jon Mulholland
Office (SOM Beckman Center, B050A): (650) 725-7532
Email: jwm at stanford.edu
Shriram Center Faculty Advisor | Dr. Alex Dunn
| Name | Role | Phone | Location | |
| Cedric Espenel |
Shriram CSIF manager, advanced imaging specialist |
650-498 7409 | espenel at stanford.edu | B023 |
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Location |
Hours |
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Shriram Center for Bioengineering & Chemical Engineering Stanford University School of Engineering Quad 443 Via Ortega B023 Cell Sciences Imaging Facility Stanford, CA 94305 |
8 a.m. - 5 p.m., Monday to Friday 24/7 access for trained users
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As with all Stanford Service Centers, credit must be given to Cell Sciences Imaging Facility for data that results in a publication. If the work done at Cell Sciences Imaging Facility produces data resulting in a figure in a publication, you are required to acknowledge Cell Sciences Imaging Facility in the publication. Further, if Cell Sciences Imaging Facility staff members provided significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the PI), then it is expected that these individuals will be coauthors on the publication.
Additionally almost all of the CSIF's microscopes were obtained via NIH instrumentation grants and therefore require proper acknowmedgement. Please see the required NIH citation information included on the microscope's description page or contact the CSIF director.
| Name | Role | Phone | Location | |
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| Jon Mulholland |
Director
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650.725.7532
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jwm@stanford.edu
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Beckman B050
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| Cedric Espenel |
Manager
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650.498.7409
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espenel@stanford.edu
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Shriram B023
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| Price List |
| ► Equipment / Resources (4) | |||
| Name | Description | Price | |
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| Axioimager Epifluorescence Widefield | Inquire | ||
| LSM780 Confocal/Multiphoton | (view additional details) | Inquire | |
| Nikon Spinning Disk Confocal | (view additional details) | Inquire | |
| Work Station - Imaris | (view additional details) | Inquire | |
| ► Services and Training (3) | |||
| Name | Description | Price | |
| Bruker Resolve Bio-AFM training | (view additional details) | Inquire | |
| Microscope Training & Usage Set-Up | (view additional details) | Inquire | |
| Technical Assistance / Consulting (per hour) | (view additional details) | Inquire | |
| ► Supplies (8) | |||
| Name | Description | Price | |
| Bioptech chamber | Inquire | ||
| Fluoro Dish 23mm (small) | Inquire | ||
| Fluoro Dish 40mm (large) | Inquire | ||
| High performance #1.5 cover glass (100/box) | (view additional details) | Inquire | |
| Materials | Inquire | ||
| Mattek Chamber | Inquire | ||
| Nunc Chamber | Inquire | ||
| POC-R coverslip | Inquire | ||
| ► Equipment / Resources (5) | ||||||||||||||||||
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