Cell Sciences Imaging Facility: Shriram Center

 

Overview of Services

 
 

 

Welcome to the Cell Sciences Imaging Facility at the Shriram Center

 

The Cell Sciences Imaging Facility (CSIF) is a University service center that provides high resolution, state-of-the-art technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials.  The CSIF operates two sites at Stanford University: The CSIF at SOE Shriram center and the CSIF at SOM Beckman cent.  Both facility sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval).  You are on CSIF's Shriram iLab Solutions site.

 

The CSIF at Shriram provides access to and training on advanced fluorescence and transmitted light microscopy including: laser scanning spectral confocal with AiryScan, multi-photon imaging (Zeiss LSM780, AiryScan, DeepSee), spinning disk confocal microscope with TIRF and photo-activation capabilities (Nikon SDC), fluorescence lifetime imaging (B&H FLIM), Lattice Lightsheet(Zeiss LLS7), lightsheet (Bruker TrueLive) and widefield (epi-fluorescence, Zeiss AxioImager) microscopes.  Detailed information on these microscopes can be found in the Schedule Equipment tab above.  Quick links to the microscopes scheduling calenders are below.

 

The CSIF at Beckman houses both light and electron microscopy as well as higly-multiplex spatial proteomic services. Light microscopy systems: Upright and inverted confocal laser scanning systems (Leica Stellaris8 DIVE upright; Zeiss LSM880 inverted, AiryScan, AiryScan FAST; Leica SP8 with fast resonant scanning, upright LEICA Stellaris 8 DIVE with fast resonant scanning) and structured illumination super resolution (GE OMX SIM, TIRF) are accessible by users.  FESEM (Zeiss, Sigma) and TEM (JEOL1400) are available with our full service EM facility. Addtionally highly-multiplex spatial proteomic services, which include Akoya CODEX Phenocycler and Array tomography services are available.  Click this link to access CSIF Beckman Center iLab site. 

 

Both facilities house data processing software for 3D volume rendering and image analysis (VisioPharm, Bitplane Imaris, PerkinElmer Volocity, SVI Huygens Deconvolution, ImageJ FIJI). Training, as well as assistance with experimental design, is available on all key technologies.

 

 

The CSIF is a member of and is supported by:

The SOM Beckman Center, Stanford Cancer Institute, BioX and the SOE

 

Getting Started

  • Stanford Users:  LOGIN or REGISTER for an iLab account using valid Stanford credentials (SUNet ID).

 

Leadership

 

CSIF Director | Jon Mulholland 

Office (SOM Beckman Center, B050A):  (650) 725-7532

Email:  jwm at stanford.edu

 

Shriram Center Faculty Advisor | Dr. Alex Dunn 

 

Staff

Name Role Phone (650) Email  Location
David Lenzi Shriram Center CSIF Manager 498-7409 dlenzi@stanford.edu Shriram B023
Nadia Makarova Shriram Bio-AFM support  497-6443 nmak@stanford.edu Shriram B023

Location

Hours

Shriram Center for Bioengineering & Chemical Engineering

443 Via Ortega

room B023, Cell Sciences Imaging Facility

Stanford, CA 94305

8 a.m. - 5 p.m., Monday to Friday  

24/7 access for trained users

 

 

 

Reservation Calendars Quick Links

    

Publication Acknowledgement 

As with all Stanford Service Centers, credit must be given to Cell Sciences Imaging Facility for data that results in a publication. If the work done at Cell Sciences Imaging Facility produces data resulting in a figure in a publication, you are required to acknowledge Cell Sciences Imaging Facility in the publication. Further, if Cell Sciences Imaging Facility staff members provided significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the PI), then it is expected that these individuals will be coauthors on the publication.

 

Additionally, almost all of the CSIF's microscopes were obtained via NIH instrumentation grants and therefore require proper acknowmedgement. Please see the required NIH citation information included on the microscope's description page or contact the CSIF director.

 

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Contacts

Name Role Phone Email Location
Jon Mulholland
Director of CSIF
 
650-725 7532
 
jwm@stanford.edu
 
Beckman B050A
 
David Lenzi
CSIF Shriram Manager
 
650 498 7409
 
dlenzi@stanford.edu
 
Shriram B023
 
Nadia Makarova
CSIF bio-AFM Specialist
 
650-497 6443
 
nmak@stanford.edu
 
Shriram B023
 

Price List


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Available Equipment and Resources

Equipment / Resources (5)

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