Welcome!
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Welcome to the Cell Sciences Imaging Facility at the Shriram Center
The Cell Sciences Imaging Facility (CSIF) is a University service center that provides high resolution, state-of-the-art technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials. The CSIF operates two sites at Stanford University: The CSIF at SOE Shriram center and the CSIF at SOM Beckman cent. Both facility sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval). You are on CSIF's Shriram iLab Solutions site.
The CSIF at Shriram provides access to and training on advanced fluorescence and transmitted light microscopy including: laser scanning spectral confocal with AiryScan, multi-photon imaging (Zeiss LSM780, AiryScan, DeepSee), spinning disk confocal microscope with TIRF and photo-activation capabilities (Nikon SDC), fluorescence lifetime imaging (B&H FLIM) and widefield (epi-fluorescence, Zeiss AxioImager) microscopes. Detailed information on these microscopes can be found in the Schedule Equipment tab above. Quick links to the microscopes scheduling calenders are below.
The CSIF at Beckman houses both light and electron microscopy as well as higly-multiplex spatial proteomic services. Light microscopy systems: Upright and inverted confocal laser scanning systems (Leica Stellaris8 DIVE upright; Zeiss LSM880 inverted, AiryScan, AiryScan FAST; Leica SP8 with fast resonant scanning, upright LEICA Stellaris 8 DIVE with fast resonant scanning) and structured illumination super resolution (GE OMX SIM, TIRF) are accessible by users. FESEM (Zeiss, Sigma) and TEM (JEOL1400) are available with our full service EM facility. Addtionally highly-multiplex spatial proteomic services, which include Akoya CODEX Phenocycler and Array tomography services are available. Click this link to access CSIF Beckman Center iLab site.
Both facilities house data processing software for 3D volume rendering and image analysis (VisioPharm, Bitplane Imaris, PerkinElmer Volocity, SVI Huygens Deconvolution, ImageJ FIJI). Training, as well as assistance with experimental design, is available on all key technologies.
The CSIF is a member of and is supported by: The SOM Beckman Center, Stanford Cancer Institute, BioX and the SOE
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CSIF Director | Jon Mulholland
Office (SOM Beckman Center, B050A): (650) 725-7532
Email: jwm at stanford.edu
Shriram Center Faculty Advisor | Dr. Alex Dunn
| Name | Role | Phone (650) | Location | |
| David Lenzi | Shriram Center CSIF Manager | 498-7409 | dlenzi@stanford.edu | Shriram 023 |
| Alana P. Gudinas | Shriram Bio-AFM super user / Bio-AFM support | agudinas@stanford.edu | Shriram 023 |
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Location |
Hours |
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Shriram Center for Bioengineering & Chemical Engineering 443 Via Ortega room B023, Cell Sciences Imaging Facility Stanford, CA 94305 |
8 a.m. - 5 p.m., Monday to Friday 24/7 access for trained users
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As with all Stanford Service Centers, credit must be given to Cell Sciences Imaging Facility for data that results in a publication. If the work done at Cell Sciences Imaging Facility produces data resulting in a figure in a publication, you are required to acknowledge Cell Sciences Imaging Facility in the publication. Further, if Cell Sciences Imaging Facility staff members provided significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the PI), then it is expected that these individuals will be coauthors on the publication.
Additionally, almost all of the CSIF's microscopes were obtained via NIH instrumentation grants and therefore require proper acknowmedgement. Please see the required NIH citation information included on the microscope's description page or contact the CSIF director.
| Name | Role | Phone | Location | |
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| Jon Mulholland |
Director of CSIF
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650-725-7532
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jwm@stanford.edu
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Beckman B050A
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| David Lenzi |
CSIF Shriram Manager
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650 498 7409
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dlenzi@stanford.edu
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Shriram B023
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| Alana Gudinas |
CSIF bio-AFM contact
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agudinas@stanford.edu
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Shriram B023
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| Price List |
| ► Equipment / Resources (4) | |||
| Name | Description | Price | |
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| Axioimager Epifluorescence Widefield | (view additional details) | Inquire | |
| LSM780 Confocal/Multiphoton | (view additional details) | Inquire | |
| Nikon Spinning Disk Confocal | (view additional details) | Inquire | |
| Work Station - Imaris | (view additional details) | Inquire | |
| ► Services and Training (3) | |||
| Name | Description | Price | |
| Bruker Resolve Bio-AFM training | (view additional details) | Inquire | |
| Microscope Training & Usage Set-Up | (view additional details) | Inquire | |
| Technical Assistance / Consulting (per hour) | (view additional details) | Inquire | |
| ► Supplies (8) | |||
| Name | Description | Price | |
| Bioptech chamber/Glass-bottom 40mm Willco dish | Inquire | ||
| Fluoro Dish 23mm (small) | Inquire | ||
| Fluoro Dish 40mm (large) | Inquire | ||
| High performance #1.5 cover glass (100/box) | (view additional details) | Inquire | |
| Materials | Inquire | ||
| Mattek Chamber | Inquire | ||
| Nunc Chamber | Inquire | ||
| POC-R coverslip | Inquire | ||
| ► Equipment / Resources (5) | ||||||||||||||||||
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