Cell Sciences Imaging Facility: Shriram Center

Welcome to the Cell Sciences Imaging Facility at the Shriram Center

The Cell Sciences Imaging Facility (CSIF) is a University service center that provides high resolution, state-of-the-art technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials.  The CSIF operates two sites at Stanford University: The CSIF at SOE Shriram center and the CSIF at SOM Beckman Center.  Both facility sites are open to all members of the Stanford community as well as to external academic and industry researchers (with approval).  You are on CSIF's Shriram iLab Solutions site.

The CSIF at Shriram provides access to and training on advanced fluorescence and transmitted light microscopy including: laser scanning spectral confocal with AiryScan, multi-photon imaging (Zeiss LSM780, AiryScan, DeepSee), spinning disk confocal microscope with TIRF and photo-activation capabilities (Nikon SDC), fluorescence lifetime imaging (B&H FLIM), Lattice Lightsheet(Zeiss LLS7), lightsheet (Bruker TrueLive) and widefield (epi-fluorescence, Zeiss AxioImager) microscopes.  Detailed information on these microscopes can be found in the Schedule Equipment tab above.  Quick links to the microscopes scheduling calenders are below.

The CSIF at Beckman houses both light microscopy (including higly-multiplex spatial proteomic services), data analysis (Huygens, Imaris, and other opensource options) software and electron microscopy services and instrumentations. Light microscopy systems: Upright and inverted confocal laser scanning systems (Leica Stellaris8 DIVE upright Multiphoton; Zeiss LSM880 inverted, AiryScan, AiryScan FAST; Leica SP8 with 8000Hz resonant scanning, upright LEICA Stellaris 8 DIVE with 8000Hz resonant scanning) and structured illumination super resolution (Leica OMX SIM, TIRF) are accessible by users.  FESEM (Zeiss, Sigma) and TEM (JEOL1400) are available with our full service EM facility. Addtionally highly-multiplex spatial proteomic services, which include Akoya CODEX Phenocycler-Fusion 2.0, Visiopharm analysis are available.  Click this link to access CSIF Beckman Center iLab site. 

Both facilities house data processing software for 3D volume rendering and image analysis (VisioPharm, Bitplane Imaris, PerkinElmer Volocity, SVI Huygens Deconvolution, ImageJ FIJI). Training, as well as assistance with experimental design, is available on all key technologies.

The CSIF is a member of and is supported by:

The SOM Beckman Center, Stanford Cancer Institute, BioX and the SOE