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(hide this warning on this page)HTS @ The Nucleus RRID: SCR_023235
The High-Throughput Screening @ The Nucleus's mission is to provide researchers at Stanford with the ability to run high-throughput chemical, siRNA, cDNA, and high-content screens for the purpose of drug and/or target discovery. The HTS @ The Nucleus is a Sarafan ChEM-H Stanford University core facility and was originally created in 2003 by the Department of Chemical and Systems Biology (formerly Molecular Pharmacology). The HTS @ The Nucleus is a shared resource (Bioscience Screening Facility) for the Stanford Cancer Institute.
This high-throughput screening (HTS) laboratory allows Stanford researchers and others to discover novel modulators of targets that otherwise would not be practical in industry. The center incorporates instrumentation (purchased with NCRR NIH Instrumentation grant numbers S10RR019513, S10RR026338, and S10OD026899), databases , compound libraries , and personnel whose previous sole domains were in industry.
Among our instrumentation are a fully automated Thermo Integrated Robotic Screening System which includes a Beckman Echo 655 acoustic dispenser for compound transfers, a fully automated Molecular Devices ImageXpress Micro Confocal High-Content fluorescence microplate imager, with live cell, fluidics and transmitted light options, and Agilent Bravo microplate liquid handlers, and BMG Clariostar Pluses, Tecan Infinite M1000 and M1000 PRO, and Molecular Devices FlexStation II 384 fluorescence, luminescence and absorbance multimode microplate readers.
We have over 225,000 small molecules for compound screens, 15,000 cDNAs for genomic screens, and whole genome siRNA libraries targeting the human genome (the siARRAY whole human genome siRNA library from Dharmacon, targeting 21,000 human genes) and the mouse genome (Qiagen mouse whole genome siRNA set V1 against 22,124 genes).
Bruce Koch, Ph.D. | HTS @ The Nucleus Director
Phone: (650) 724-1835
Email: bkoch at stanford.edu
David E. Solow-Cordero, Ph.D. | HTS @ The Nucleus Associate Director
Phone: (650) 725-6002
Email: desolow at stanford.edu
Professor James K. Chen, Ph.D. | Faculty Advisor Co-Director HTS @ The Nucleus
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Location |
Hours |
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High-Throughput Screening @ The Nucleus Stanford University Sarafan ChEM-H W008 290 Jane Stanford Way Stanford, CA 94305
HTS CCSR lab Stanford University Medical Center CCSR Room 0133 - North Wing 269 Campus Dr. Stanford, CA 94305-5174
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9 a.m. - 6 p.m., Monday to Friday 24/7 access for trained users
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The HTS labs are located in Room W008 of the Sarafan ChEM-H building and Room 0133 (basement) of the CCSR building North Wing-Green Walls (269 Campus Dr. West).
Directions | Campus Map | Parking Map; Metered parking spots in L-16 & L-17, free parking anywhere after 4 PM.
Visit our core website for more information: https://hts.stanford.edu/
As with all Stanford Service Centers, credit must be given to Sarafan ChEM-H High-Throughput Screening @ The Nucleus for data that results in a publication. If the work done at High-Throughput Screening @ The Nucleus produces data resulting in a figure in a publication, you are required to acknowledge HTS @ The Nucleus in the publication. Further, if HTS @ The Nucleus staff members provided significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the PI), then it is expected that these individuals will be coauthors on the publication.
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| David Solow-Cordero, Ph.D. |
Associate Director, HTS @ The Nucleus
|
650 725-6002
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desolow@stanford.edu
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CCSR 0133A
|
| Jason Wu |
HTS CCSR Research Assistant
|
(650) 725-6004; Lab: (650) 736-7638; HT Imaging Rm: (650) 736-0084
|
jjwu@stanford.edu
|
CCSR 0133
|
| Bruce Koch |
Director, HTS @ The Nucleus
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650-724-1835
|
bkoch@stanford.edu
|
ChEM-H W008
|
| Oliver Hamto |
HTS Automation Specialist
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(650) 497-8018
|
ohamto@stanford.edu
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ChEM-H W008
|
| Corey Liu |
ChEM-H Operations
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(650) 724-7445
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liuc@stanford.edu
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ChEM-H N135D
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| Chris Caridi |
HCS Scientist
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(650) 725-6002
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ccaridi@stanford.edu
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CCSR 0133A
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| Fixed Cost Services |
| ► Consulting Services (3) | |||
| Name | Description | Price | |
|---|---|---|---|
| Consulting - Assay Development (per hour) | (view additional details) |
External Non-profit / International Academic (with PTA)
$115.00
each
External For Profit (with PTA) $345.00 each |
|
| Consulting - Database (per hour) | (view additional details) |
External Non-profit / International Academic (with PTA)
$150.00
each
External For Profit (with PTA) $450.00 each |
|
| Consulting - Laboratory Technician (per hour) | (view additional details) | Inquire | |
| ► High-Throughput Screening Services (3) | |||
| Name | Description | Price | |
| Assay Development (per HTS) | (view additional details) |
External Non-profit / International Academic (with PTA)
$0.00
each
|
|
| Compound Libraries Fee (per HTS) | (view additional details) |
External For Profit (with PTA)
$8,000.00
each
External Non-profit / International Academic (with PTA) $4,000.00 each |
|
| HTS Database (per HTS) | (view additional details) |
External Non-profit / International Academic (with PTA)
$4,000.00
each
External For Profit (with PTA) $8,000.00 each |
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| ► Integrated Robotic Systems (3) | |||||||||||||||||||||||||||||||||||||||||||||
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| ► Liquid Handling Systems (6) | |||||||||||||||||||||||||||||||||||||||||||||
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