Translational Applications Service Center

Overview of Services

 

                                                                         TASC Services

 

Translational Applications Service Center (TASC) facility is a fee-for-service research laboratory serving the Stanford University scientific community, as well as outside investigators. It offers a variety of analytical services, technical and scientific consulting and training, as well as research equipment, which allow investigators shared access to technologies that support innovative research in translational medicine. By providing centralized oversight and infrastructure for conducting translational research studies, as well as access to high-quality, cost-efficient, state-of-the-art proteomic, molecular and genomic technologies, TASC's overall mission is to serve as a comprehensive resource for accelerating the pace of advances in patient therapy and diagnosis by enhancing basic research in the early stages of translation to the clinical setting.

 

TASC provides a wide variety of analytical services, including a unique state-of-the art novel proteomics technology, NanoImmunoAssay (NIA), for defining protein signatures and measuring proteomic response to targeted therapies in rare clinical samples. Our facility offers dedicated research staff to facilitate the ability of clinical researchers to employ novel technologies and scientific discoveries at the point of care. Of critical importance, our facility also provides users with initial training and on-site expertise in the proper use of the equipment and procedures to enable collection of meaningful, high quality data. Additionally, TASC offers consulting services to assist in experimental design and data analysis, to take best advantage of the available resources.

 

We can provide scientific expertise and support for standard and customized assays, from the initial study design to the final interpretation and statistical analysis of experimental data. Our standard services also include clinical sample acquisition, processing and archival for conducting inter-disciplinary translational research applications.

 

Leadership

 

Joanna Liliental

TASC Director

Map

 Campus Map

Location and hours of operation

Hours Location

Monday - Friday  

9:00 - 5:00  

Grant Building S-107
300 Pasteur Drive, Grant, S-107
Stanford, CA 94305-5110

 

 

Publication Acknowledgement

As with all Stanford Service Centers, credit must be given to the TASC Facility for use that results in a publication.

If the work done at the TASC Service Center produces data resulting in a figure in a publication, you are required to acknowledge the Stanford TASC Facility in the publication. Per university policy regarding the shared services, investigators who use TASC are to include the following verbiage in the their manuscript: “We thank the Stanford TASC Facility and it's Director Joanna Liliental for for assistance with this research.”

Further, if the TASC Director/ staff members provided significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the PI), then it is expected that these individuals will be coauthors on the publication.

If the TASC Director/staff members made a significant contribution to a publication such as generating data for a figure, then it is expected that these individuals should have coauthorship (as evaluated by the PI).

 

Links and Resources

  1. TASC Website
  2. Nano-Immuno Assay Overview
  3. Directions and Parking

Contacts

Name Role Phone Email Location
Joanna Liliental, PhD
TASC Director
 
650.736.1285
 
jlili@stanford.edu
 
Grant S 107
 
ShailjaPatel
Lab Manager
 
650-736-1285
 
baroda at stanford.edu
 
Grant S107
 

Service List


Search available services:
Name Description Price
01. Technical Assistance with assay set-up/analysis, Consulting or Training

Note regarding Training:

Anyone interested in independent usage of the PeggySue or Milo equipment must complete a mandatory 4 hour training session by TASC staff.

TASC will train any high-volume user wishing to operate the equipment independently ONLY if

the project requires using the instrument on a regular basis (ie: at least once a week) for more than 3 months.

 

Note about ELISA assay rates:

Rate is cost of the ELISA kit + labor (technical assistance with assay set-up and analysis).

Rates can be subject to change, depending on the assay and the amount of technical assistance it requires.

Here are general, typical rates for most ELISA assays (assuming samples are ran in duplicates):

 

1-8 samples: 2.5 hrs (ie: 2-16 sample wells+ 16 standard curve wells)

9-16 samples: 3hrs (ie: 17-32 sample wells+ 16 standard curve wells)

17-24 samples: 4 hrs (ie: 33-48 sample wells+ 16 standard curve wells)

25-40 samples (in duplicate): 5 hrs (49-80 sample wells + 16 standard curve wells)

 

One full 96 well plate= 80 samples wells (40 samples in duplicate)  + 16 standard curve wells)

Inquire
02. NIA Charge Separation Assay - full service (per cycle)

One cycle is defined as an assay array of 12 sample wells x 1 target antibody

 

  • Assay Development and optimization, set-up, data analysis
  • PeggySue instrument use, initial consultation, labor, consumables/reagents are included, with the exception of primary antibodies, which are user's responsibility.
Inquire
03. NIA Size Separation Assay - full service (per cycle)

One cycle is defined as an assay array of 11 sample wells (+ one well of size ladder) x 1 target antibody

  • Assay Development and optimization, set-up, data analysis
  • PeggySue instrument use, initial consultation, labor, consumables/reagents are included, with the exception of primary antibodies, which are user's responsibility.
Inquire
04. NIA Charge or Size Assay - Instrument Use Fee for PeggySue

FOR INDEPENDENT USERS SELF-OPERATING THE PeggySue INSTRUMENT

 

Anyone interested in independent usage of the PeggySue equipment must complete a mandatory 4 hour training session by TASC staff.

This is a prerequisite for all independent usage of the PeggySue equipment in the facility.

TASC will train any high-volume user wishing to operate the equipment independently ONLY if

the project requires using the PeggySue on a regular basis (ie: at least once a week) for more than 3 months.

Inquire
05. NIA Charge Assay - Materials at cost Inquire
06. NIA size Assay - Materials at cost (or NIA charge assay with amplification) Inquire
07. Genomic DNA Extraction/Processing (QiaAmp)

Per sample

Inquire
08. RNA Extraction/Processing (Rneasy Plus)

Per Sample

Inquire
09. RNA or DNA Extraction/Processing (FFPE)

Per Sample

Inquire
10. EMULATE OOC (organ on chip) instrument daily use

Per day use of Emulate ZOE/ORB

Internal Stanford $137.00 each
External For Profit (with PTA) $191.80 each
External Non-profit / International Academic (no PTA) $215.64 each
External Domestic Academic (with PTA) $137.00 each
External Non-profit / International Academic (with PTA) $137.00 each
External For Profit (no PTA) $301.89 each
External Domestic Academic (no PTA) $215.64 each
TRAM Member $123.00 each
External Affiliates (no PTA) $147.96 each
External Affiliates (with PTA) $137.00 each
CZ Biohub (with PTA) $137.00 each
12. PBMC Processing (Ficoll)

Per Blood Sample

Inquire
13. PBMC Processing (Buffy Coat)

Per Sample

Inquire
14. Serum or Plasma Processing

Per Sample

Inquire
15. Tissue/cells lysis - protein isolation

1-5 samples

Inquire
16. Tissue Homogenization (TissueLyserLT)

Per Sample

Inquire
17. DNA/RNA Yield and Purity Determination (NanoDrop)

Per Sample

Inquire
18. Protein Concetration Assay (Lowry)

Per Sample

Inquire
19. Cryopreservation of cells (DMSO)

Per Sample

Inquire
21. Sample Bio-Banking: -80C Freezer Storage (per box per month)

-80C Freezer Storage (per box per month)

Inquire
22. Sample Bio-Banking: liquid nitrogen storage (per vial per month)

Per Vial Per Month

Inquire
24. MILO assay - PAY PER USE

MILO instrument use fee per assay per chip.

 

User is responsible for reagents, consumables, chip scanning (can be arranged through SFGF Facility) and data analysis.

(Chip scanning and data analysis can be included, but additional fees will apply).

Inquire
25. MILO assay - FULL SERVICE

MILO assay - single cell Western (full service)

 

Includes instrument use, labor to run one assay, Milo kit, which includes reagents and consumables to run one assay (1 chip, secondary antibodies and kit reagents to run one assay, excluding the primary antibody).

DOES NOT INCLUDE DATA ANALYSIS (Chip scanning and data analysis can be included, but additional fees will apply)

DOES NOT INCLUDE PRIMARY ANTIBODIES (User is responsible for providing the primary antibodies)

Inquire
26. EMULATE Training and new user onboarding

EMULATE Organ on Chip (OOC) Training and onboarding

Internal Stanford $1,000.00 each
External Domestic Academic (with PTA) $1,000.00 each
External For Profit (with PTA) $1,400.00 each
External Non-profit / International Academic (with PTA) $1,000.00 each
External For Profit (no PTA) $2,203.60 each
External Non-profit / International Academic (no PTA) $1,574.00 each
External Domestic Academic (no PTA) $1,080.00 each
External Affiliates (no PTA) $1,080.00 each
External Affiliates (with PTA) $1,000.00 each
TRAM Member $900.00 each
(unused1) External Academic not Univ funded (with PTA) $0.00 each
(unused2) External Academic not Univ funded (without PTA) $0.00 each
CZ Biohub (with PTA) $1,000.00 each