Viruses Produced
![]() Adeno-associated viruses Lentiviruses Retroviruses |
Overview of Services
The delivery of recombinant genes into neurons is a critically important strategy for understanding the molecular mechanisms underlying all brain functions, as well as for how these mechanisms go awry in brain disorders. A complementary and equally important strategy is delivery of inhibitory RNAs to eliminate or reduce specific brain proteins. Genetically engineered viruses provide powerful tools for introducing these constructs into brain cells. Indeed it is now possible, using a single virus particle, to both eliminate specific proteins and replace them with modified versions in specific subsets of cells in the brain. It is also possible, using viruses, to express proteins that will allow precise control over the electrical activity of individual nerve cells. These virally mediated molecular manipulations allow unprecedented experimental control over synapses, cells and circuits in model systems as well as in vivo in the mammalian brain.
To facilitate the use of these state-of-the-art methodologies by Stanford neuroscientists, the Neuroscience Gene Vector and Virus Core centralizes the process of producing and distributing viral vectors and cDNA plasmids. This benefits the Wu Tsai Neurosciences Institute’s overall mission by preventing the duplication of efforts by Stanford faculty and thus greatly increasing the efficiency of all of the Wu Tsai Neurosciences Institute’s programs. Stock VirusesThe virus core has generated several pre-made viruses (AAVs, lentiviruses, retroviruses) that are available at cost on a first come, first served basis. Stock viruses can serve several purposes such as:
Since the stock viruses are pre-made, the time required to obtain them is significantly shortened. Several stock viruses are currently available that express fluorescent reporters or optogenetic tools. Stock viruses with specific genes of interest will be generated and maintained if there is enough ongoing demand.
List of currently available stock viruses: https://neuroscience.stanford.edu/research/programs/community-labs/neuroscience-gene-vector-and-virus-core/stock-viruses |
To request any service from our core, please read and follow the instructions in the forms below:
Form to set up iLAB and place orders for Stanford users
External users:
Form to set up iLAB and place orders for external users
Javier F. Alcudia, PhD | Director
Office: S081
Phone: 650-723-0588
Email: jalcudia at stanford dot edu
Location |
Hours |
Gene Vector and Virus Core, Stanford University Wu Tsai Neurosciences Institute
290 Jane Stanford Way Stanford CA 94305
LAB room S078
Mail Address: Wu Tsai Neurosciences Institute
290 Jane Stanford Way Stanford CA 94305
MC5088
Subbu Dhulipala (stock virus) Lab Phone: 650-724-8451 Email: sri2 at stanford dot edu
Monika Avina Lab Phone: 650-724-8451 monika.avina at stanford dot edu
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9:00 a.m. - 6 p.m., Monday to Friday
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As with all Stanford Service Centers, credit must be given to Neuroscience Gene Vector and Virus Core for data that results in a publication. If the work done at GVVC produces data resulting in a figure in a publication, you are required to acknowledge Neuroscience Gene Vector and Virus Core in the publication. Further, if staff members provided significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the PI), then it is expected that these individuals will be coauthors on the publication.
Name | Role | Phone | Location | |
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Javier F. Alcudia, PhD |
GVVC Director
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650.723.0588
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jalcudia@stanford.edu
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290 Jane Stanford Way, S081
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Maritza Vallejo |
Financial Contact
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maritza.vallejo@stanford.edu
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290 Jane Stanford Way, E152
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Subbu Dhulipala |
GVVC Staff
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650-724-8451
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sri2@stanford.edu
|
290 Jane Stanford Way, S078
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Monika Avina |
GVVC Staff
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650-724-8451
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skulkar2@stanford.edu
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290 Jane Stanford Way, S078
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Service Price List |
Name | Description | Price |
---|---|---|
GVVC-AAV-165 | AAV-DJ-Ef1a-fDIO GCaMP 6s - per ul; MTA-D req. | Inquire | |
GVVC-AAV-193 | AAV-8- CaMKIIa-ChRmine-mScarlet-WPRE - per uL; MTA-D req. | Inquire | |
GVVC-AAV-197 | AAV-8- nEF-Con/Fon TVA-mCherry WPRE - per uL; MTA-D req. | Inquire |