Gene Vector and Virus Core

 

 

 

Welcome to the GVVC - Gene Vector and Virus Core

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Viruses Produced

 

Adeno-associated viruses 
Adeno-associated viruses

 

Lentiviruses

Lentiviruses

 

Retroviruses

Retroviruses

Overview of Services 

 

The delivery of recombinant genes into neurons is a critically important strategy for understanding the molecular mechanisms underlying all brain functions, as well as for how these mechanisms go awry in brain disorders. A complementary and equally important strategy is delivery of inhibitory RNAs to eliminate or reduce specific brain proteins. Genetically engineered viruses provide powerful tools for introducing these constructs into brain cells. Indeed it is now possible, using a single virus particle, to both eliminate specific proteins and replace them with modified versions in specific subsets of cells in the brain. It is also possible, using viruses, to express proteins that will allow precise control over the electrical activity of individual nerve cells. These virally mediated molecular manipulations allow unprecedented experimental control over synapses, cells and circuits in model systems as well as in vivo in the mammalian brain.

 

To facilitate the use of these state-of-the-art methodologies by Stanford neuroscientists, the Neuroscience Gene Vector and Virus Core centralizes the process of producing and distributing viral vectors and cDNA plasmids. This benefits SINTN’s overall mission by preventing the duplication of efforts by Stanford faculty and thus greatly increasing the efficiency of all of SINTN’s programs.

 
 

Stock Viruses

 
The virus core has generated several pre-made viruses (AAVs, lentiviruses, retroviruses) that are available at cost on a first come, first served basis. Stock viruses can serve several purposes such as:
  • negative controls
  • screening cells and animals for infection efficiency in cases where it is unknown (“tropism screening”)
  • practicing infections or in vivo injections.

 

Since the stock viruses are pre-made, the time required to obtain them is significantly shortened. Several stock viruses are currently available that express fluorescent reporters or optogenetic tools. Stock viruses with specific genes of interest will be generated and maintained if there is enough ongoing demand.

 

List of currently available stock viruses:

http://med.stanford.edu/gvvc/stockviruses.html

 

 

Getting Started - Account Setup

 

  • Stanford Users:  LOGIN or REGISTER for an iLab account using valid Stanford credentials (SUNet ID).

 

 

 

 

To request any service from our core, please read and follow the instructions in the forms below:

 

 

 

 

 

Before Beginning a Project

 
Researchers are strongly encouraged to contact and interact with the Gene Vector and Virus Core as early in a project as possible. Many of the projects that fail are a result of mistakes (usually in vector design or characterization) that could be avoided if the virus core was consulted at an early stage. The virus core does not charge for advice or consultations.
 
The following should always be performed during the course of producing and using recombinant viruses. Click here for a downloadable format of checklist.

  • Obtain institutional approval to use recombinant viruses.
  • Choose virus.
  • Design vector.
  • Provide virus core with predicted sequence of vector.
  • Construct vector.
  • Characterize vector.
  • Produce plasmid vector.
  • Decide how much virus to produce at what purity level.
  • Produce virus.
  • Store virus properly.
  • Use virus properly.
  • Dispose of unused virus properly.
 
Use and (disposal) of recombinant viruses is governed by specific institutional guidelines that are often based on the CDC/NIH (biosafety level/BSL) guidelines for their use. At Stanford, researchers must obtain approval to use recombinant viruses by submitting an application to the APB (Administrative Panel for Biosafety). The virus core is required to obtain approved copies of APB applications before it can produce viruses for Stanford customers. See http://www.stanford.edu/dept/EHS/prod/researchlab/bio/index.html or eProtocol.
 
 

Leadership

 

Javier F. Alcudia, PhD | Director

Office:  2135

Phone: 650-723-0588

Email:  jalcudia at stanford.edu

 

 

Location and Hours of Operation

 

Location

Hours

Gene Vector and Virus Core, Stanford University

3165 Porter Drive, Room 2216

Mail Address:

3165 Porter Drive - MC5505


Stanford, CA 94304

 

Poornima Murthi

Lab Phone: 650-724-8451

Email:  pmurthi at stanford.edu

 

 

 

9:30 a.m. - 7 p.m., Monday to Friday  

 

 

 

  

Visitor Information

 

The Neuroscience Gene Vector and Virus Core is located in the Stanford University School of Medicine Technology & Innovation Park at Porter Drive south of the main campus.

The virus core lab is in 3165 Porter Drive on the main floor in room 2216.

 

Marguerite Shuttle bus: Lines 1050A, R and RP

http://transportation.stanford.edu/marguerite/schedules.php

 

Links and Resources

 

  • Working with viral vectors: MSDS

 

    

Publication Acknowledgement 

 

As with all Stanford Service Centers, credit must be given to Neuroscience Gene Vector and Virus Core for data that results in a publication. If the work done at GVVC produces data resulting in a figure in a publication, you are required to acknowledge Neuroscience Gene Vector and Virus Core in the publication. Further, if staff members provided significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the PI), then it is expected that these individuals will be coauthors on the publication.

 

 

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Contacts

Name Role Phone Email Location
Javier F. Alcudia, PhD
GVVC Director
 
650.723.0588
 
jalcudia@stanford.edu
 
3165 Porter Drive Rm 2135
 
Poornima Murthi
GVVC Staff (custom productions)
 
650-724-8451
 
pmurthi@stanford.edu
 
3165 Porter Drive Rm 2216
 
Catherine Lau
Financial Contact
 

 
cathylau@stanford.edu
 

 
Subbu Dhulipala
GVVC Staff (Stock Virus)
 
650-724-8451
 
sri2@stanford.edu
 
3165 Porter Drive Rm 2216
 
Haritha Duggireddy, PhD
GVVC Staff (custom productions)
 
650-724-8451
 
harithad@stanford.edu
 
3165 Porter Drive Rm 2216
 

FY15 Service Price List


Search available services:
Name Description Price
Cell Line Construction (polyclonal pool; virus not inc.) - per T-25 External Academic not Univ funded (without PTA) $295.63 each
GVVC-AAV-052 | AAV DJ-EF1a DIO C1V1 (E162T)-TS-p2A-mCherry-WPRE - per ul; MTA-D req. Inquire
GVVC-AAV-088 | AAV-DJ CamKIIa-GCaMP 6s - per uL; MTA-D req. Inquire
GVVC-AAV-089 | AAV-DJ CamKIIa-GCaMP 6m - - per uL; MTA-D req. Inquire
GVVC-AAV-090 | AAV-DJ CamKIIa-GCaMP 6f - per uL, MTA-D req. Inquire
GVVC-AAV-091 | AAV-DJ EF1a-DIO-GCaMP 6s - per uL; MTA-D req. Inquire
GVVC-AAV-092 | AAV-DJ EF1a-DIO-GCaMP 6m - per uL; MTA-D req. Inquire
GVVC-AAV-093 | AAV-DJ EF1a-DIO-GCaMP 6f - per uL; MTA-D req. Inquire
GVVC-AAV-094 | AAV-DJ hSyn-GCaMP 6s - per uL; MTA-D req. Inquire
GVVC-AAV-095 | AAV-DJ hSyn-GCaMP 6m - per uL; MTA-D req. Inquire
GVVC-AAV-096 | AAV-DJ hSyn-GCaMP 6f - per uL; MTA-D req. Inquire
GVVC-AAV-097 | AAV-DJ EF1a-mCherry - per uL Inquire
GVVC-AAV-098 | AAV-5 CAG-FLEx(FRT)-TC - per uL Inquire
GVVC-AAV-099 | AAV-8 CAG-FLEx(FRT)-G - per uL Inquire
GVVC-AAV-100 | pAAV hSyn FLEX-mGFP 2A Synaptophysin-mRuby - per uL Inquire
GVVC-AAV-101 | pAAV hSyn FLEX-FRT-mGFP-2A-Synaptophysin-mRuby - per uL Inquire
GVVC-AAV-102 | pAAV-pMecp2-SpCas9-spA (PX551) - per uL Inquire
GVVC-AAV-103 | pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (PX552) - per uL Inquire
GVVC-AAV-104 | pAAV-CMV--NLS-SaCas9-NLS-3xHA-bGHpA;U6--BsaI-sgRNA (PX601) - per uL Inquire
GVVC-AAV-105 | AAV-DJ CaMKIIa-GFP - per uL; MTA-D req. Inquire
GVVC-AAV-106 | AAV-8-CaMKIIa-iC++-EYFP - per uL; MTA-D req. Inquire
GVVC-AAV-107 | AAV-8-CaMKIIa-SwiChR++-EYFP - per uL; MTA-D req. Inquire
GVVC-AAV-108 | AAV-8-Ef1a-DIO iC++-EYFP - per uL; MTA-D req. Inquire
GVVC-AAV-109 | AAV-8-Ef1a-DIO SwiChR++-EYFP - per uL; MTA-D req. Inquire
GVVC-AAV-110 | AAV-8-hSyn-iC++-EYFP - per uL; MTA-D req. Inquire
GVVC-AAV-111 | AAV-8-CaMKIIa-bReaChES-TS-EYFP - per uL; MTA-D req. Inquire
GVVC-AAV-112 | AAV-8-Ef1a-DIO-bReaChES-TS-EYFP - per uL; MTA-D req. Inquire
GVVC-AAV-117 | AAV-DJ-Syn-Cre-GFP - per ul

Please, cite: Kaeser PS, et al. Cell. 2011 Jan 21;144(2):282-95. doi: 10.1016/j.cell.2010.12.029.

Inquire
GVVC-AAV-119 | AAV-DJ-CaMKIIa hChR2 (C128S/D156A)-eYFP - per uL; MTA-D req. Inquire
GVVC-AAV-120 | AAV-DJ-CaMKIIa-hChR2 (C128S/D156A)-mCherry - per uL; MTA-D req. Inquire
GVVC-AAV-121 | AAV-DJ-EF1-DIO hChR2 (C128S/D156A)-mCherry - per uL; MTA-D req. Inquire
GVVC-AAV-122 | AAV 8-hSyn1 eYFP - per ul; MTA-D req. Inquire
GVVC-AAV-123 | AAV8-CaMKIIa-eYFP-NRN - per uL; MTA-D req. Inquire
GVVC-AAV-124 | AAV-DJ-Ef1a-DIO RCaMP2 - per uL; MTA-D req. Inquire
GVVC-AAV-125 | AAV-DJ-CaMKIIa-ASAP1 - per uL Inquire
GVVC-AAV-126 | AAV-DJ-Syn-ASAP1-IRES-mRuby - per uL Inquire
GVVC-AAV-127 | AAV-hSyn-eGFP - per uL Inquire
GVVC-AAV-128 | AAV-DJ-CaMKIIa-eGFP - per uL Inquire
GVVC-AAV-129 | AAV-DJ-EF1a-DIO-hM4D(Gi)-mCherry Inquire
GVVC-AAV-130 | AAV-DJ-EF1a-DIO-hM3D(Gq)-mCherry Inquire
GVVC-AAV-131 | AAV-DJ-hSyn-HA-hM4D(Gi)-mCherry Inquire
GVVC-AAV-132 | AAV-DJ-hSyn-HA-hM3D(Gq)-mCherry Inquire
GVVC-AAV-133 | AAV-DJ-CaMKIIa-hM4D(Gi)-mCherry Inquire
GVVC-AAV-134 | AAV-DJ-CaMKIIa-hM3D(Gq)-mCherry Inquire
GVVC-AAV-135 | AAV-DJ-CaMKIIa-HA-KORD-IRES-mCitrine Inquire
GVVC-AAV-136 | AAV-DJ-hSyn-dF-HA-KORD-IRES-mCitrine Inquire
GVVC-AAV-137 | AAV-8-hSyn-eNPAC 2.0 - per uL; MTA-D req. Inquire
GVVC-AAV-138 | AAV-8-EF1a-DIO-eNPAC 2.0 per uL; MTA-D req. Inquire
GVVC-AAV-139 | AAV-8-cFos-ERT2-Cre-ERT2-PEST - per ul; MTA-D req. Inquire
GVVC-AAV-140 | AAV-8-cFos-hChR2(H134R)-eYFP-PEST - per ul; MTA-D req. Inquire
GVVC-AAV-141 | AAV-8-CaMKIIa-RCaMP2 - per ul; MTA-D req. Inquire
GVVC-lenti-15 | (VSV-G) EF1a-Luciferase-IRES-Blasticidin - 5 ul/each Inquire
GVVC-lenti-16 | (VSV-G) EF1a-Cre-PGK-Puro Inquire
GVVC-lenti-17 | (VSV-G) EF1a-Flp-PGK-Neo Inquire
Pelleted LV or RV Production - first T-75 External Academic not Univ funded (without PTA) $712.47 each
Pelleted LV or RV Production - per additional T-75 External Academic not Univ funded (without PTA) $189.03 each
Plasmid Construction - per insert Inquire
Plasmid Design Inquire
Pure AAV Production - first T-225 External Academic not Univ funded (without PTA) $1,620.71 each
Pure AAV Production - per additional T-225 External Academic not Univ funded (without PTA) $260.31 each