ALERT

In Observance of U.S. Holiday, Memorial Day, Agilent Cross Lab/ iLab Operations Software Support Help Desk will be closed during U.S. hours on Monday May 25th, 2020. We will resume regular U.S. support hours on Tuesday May 26th, 2020. EU and APAC Support will remain open during this time. For urgent matters, please add "Urgent" to the ticket/ email subject or press "1", when prompted, to escalate a call on the iLab Support phone and we will prioritize those requests first.

ALERT

Due to the COVID-19 pandemic, nearly all Stanford scientific core facilities using the iLab system have ceased lab operations.  Exceptions are the TKTC, which is offering mouse sperm and embryo cryopreservation services and the Fly Facility, which continues to make Drosophila food.  Online services, such as the Genetics Bioinformatics Service Center and new on-line data analysis options at SCi3, continue to be available.  The staffs of all of the core facilities remain available (on-line, not in-person) for discussions including experiment planning, data analysis, and manuscript preparation.

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Neuroscience Microscopy Service

This hippocampal neuron was filled during whole-cell electrophysiological recording
in a 300 µm thick acute brain slice, and, after post-hoc staining, was reconstructed
using the NMS NeuroLucida workstation. X, Y, and Z scale bars = 42 μm. Traced on
NMS's NeuroLucida workstation by Dr. Csaba Földy, Südhof lab.

Overview of Services

The NMS provides access to high-end, capital-intensive microscopy equipment that is often not available to individual labs.

 Please note that the NMS will be moving most of its facilities to the basement of the new Stanford Neurosciences Building duirng the week of March 16th.  We will be packing up the i facility during the previous week.  The LSM710 Confocal Microscope is NOT impacted by this move, and will remain in SIM1.

Available equipment includes

  • Zeiss LSM710 Confocal Microscope:
  • Zeiss LSM510 Confocal Microscope;
  • LaVision Biotec Ultramicroscope II light sheet microscope;
  • Bruker/Prairie Technologies Two-photon Microscopes: 1) In Vivo, 2) Tissue Slice
  • Whole-cell patch clamp e-phys rig (on the two-photon tissue slice microscope)
  • Zeiss AxioImager Motorized Widefield Fluorescence Microscope.
  • Two high-powered image analysis workstations:
    • Harpo, with Imaris 3D imaging and analysis software, and
    • Chico, with Neurolucida tracing software
  • NEW! Zeiss LSM980 with Airyscan 2 Confocal Microscope installed in new NMS labs in Stanford Neurosciences Building (stay tuned for training details!)

Leadership

 

Andrew Olson, Ph.D.
Director, Neuroscience Microscopy Service

 

Getting Started

 

  • Stanford Users:  LOGIN or REGISTER for an iLab account using valid Stanford credentials (SUNet ID)

 

Location and hours of operation

Hours Location

Monday - Friday

8:30 - 5:00

 24/7 Access to trained, proficient users   

Lorry Lokey Stem Cell Building, Suite G0901
265 Campus Drive West
Stanford, CA 94305-5453
Stanford Mail code: 5453

Links and Resources

  1. NMS website in Wu Tsai Neurosciences Community Labs

Contacts

Name Role Phone Email Location
Andrew Olson, Ph.D.
Director
 
650.723.8818
 
nismicro@stanford.edu