Human Immune Monitoring Center

Welcome to the HIMC - Human Immune Monitoring Center

The Human Immune Monitoring Center (HIMC) is designed to be a comprehensive source for immune monitoring services for clinical and translational studies.  We perform:   Immunoassays:  Luminex and Olink Target-96 platforms Mass Cytometry (CyTOF):  Immunophenotyping, Intracellular cytokines, Phospho-epitopes Flow Cytometry:  Immunophenotyping, Intracellular cytokines, Phospho-epitopes Genomics:  RNA/DNA extractions, Microfluidic qPCR arrays, BD Rhapsody single-cell RNAseq   Parallel coordinates view of Luminex cytokine data:                             SPADE analysis of CyTOF data:    Goals of the HIMC include: To provide standardized, state-of-the art immune monitoring assays at the RNA, protein, and cellular level. These are assays that have already been validated in the HIMC. To test and develop new technologies for immune monitoring. We are always developing new assays in the above areas, as well as testing new potential immune monitoring platforms. To efficiently archive, report, and mine data from immune monitoring studies, so as to increase the value of the data and to assist in biomarker discovery. The HIMC uses Stanford Data Miner for integration of data from the standard HIMC assays, along with de-identified clinical and demographic data. One year after release of results to an investigator, data will be released for all users of the HIMC to access, unless appealed by the investigator for reasons of still-pending publication. The HIMC was developed in 2007 by the Institute for Immunity, Transplantation, and Infection (Mark Davis, Ph.D., director) in conjunction with the Center for Clinical Immunology (C. Garrison Fathman, founder).  Our Faculty Advisor is Mark Davis.

Getting Started

• Stanford Users:  LOGIN or REGISTER for an iLab account using valid Stanford credentials (SUNet ID).

• Non-Stanford Users:  Click to LOGIN with approved iLab credentials. Or, click to SIGN UP for an iLab account.  Brief instructions to get you started:

All Non-Stanford Customers - Getting a Project Quote

Please register for an iLab account so that we can generate a quote for your project. 

To get started with iLab:  Go to http://sharedfacilities.stanford.edu to register for an iLab account. Click the blue sign-up button in the upper right corner. After you click submit, iLab Support will send you your login credentials.

 Once logged in, click on 'list all cores' on the left side of your page. Locate the Human Immune Monitoring Center, then open the Request Services tab.

To set up your project and receive a quote for services: Request Services from the “External Users” > “initiate request” button.

There are two forms to complete…

1. The User (Service) Agreement form (download, then upload the signed agreement). This is our standard user service agreement and must be in place before we can begin any work.  If you are just requesting  a quote for a Grant Proposal, you may upload the form unsigned.

 Please be sure to click “save completed form”

      2.  Complete the HIMC Service Request Form to select from our menu of services.

Select services from the menus (enter the anticipated number of samples) then click “Add selected services” to save the selections to the form.

When done completing the form, click “submit completed form” (at the bottom of the page) to save the entire form.

 Once the form is submitted, we will be able to see your project in iLab and can generate a quote for your request.

Confirming Your Project is a GO!

When you've agreed to the services listed and require no more edits from us, and you want to move forward with the work, please click the Researcher Agree button.

1. Our Standard Service Agreement will need to be signed
2. We require a company Purchase Order from your company before we can begin work.  

Please let us know if you have any questions.

Thank you, and we look forward to working with you! 

Leadership

Holden Maecker, PhD | Facility Director

Office (Rm D039, Fairchild Building):  (650) 723-1671

Email:  maecker at stanford.edu

Staff

Locations and Hours of Operation 

Links and Resources

• Visit our facility website for more information: http://himc.stanford.edu/

Publication Acknowledgement 

As with all Stanford Service Centers, credit must be given to Human Immune Monitoring Center (HIMC) for data that results in a publication. If the work done at HIMC produces data resulting in a figure in a publication, you are required to acknowledge HIMC in the publication. Further, if HIMC staff members provided significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the PI), then it is expected that these individuals will be coauthors on the publication.

One of our CyTOFs was purchased with funds from a NIH S10 Shared Instrumentation Grant (S10RR027431-01).  Please acknowledge this grant in your papers using CyTOF data.

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Contacts

Map

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