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The Human Immune Monitoring Center (HIMC) is designed to be a comprehensive source for immune monitoring services for clinical and translational studies. We perform:
Immunoassays: Luminex cytokine arrays, MesoScale Discovery assays Mass Cytometry (CyTOF): Immunophenotyping, Intracellular cytokines, Phospho-epitopes Flow Cytometry: Immunophenotyping, Intracellular cytokines, Phospho-epitopes Genomics: RNA/DNA extractions, Microarrays, Microfluidic qPCR arrays, Targeted RNAseq BioBanking: Serum, Plasma, PBMC, and Tissue processing; Sample banking and retrieval
Parallel coordinates view of Luminex cytokine data: SPADE analysis of CyTOF data:   Goals of the HIMC include:
The HIMC was developed in 2007 by the Institute for Immunity, Transplantation, and Infection (Mark Davis, Ph.D., director) in conjunction with the Center for Clinical Immunology (C. Garrison Fathman, founder). Our Faculty Advisor is Mark Davis.
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Holden Maecker, PhD | Facility Director
Office (Rm D039, Fairchild Building): (650) 723-1671
Email: maecker at stanford.edu
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Name |
Role |
Phone |
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Location |
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Yael Rosenberg-Hasson |
Technical Director, Immunoassay Mgr |
650-723-4984 |
yaelhr at stanford.edu |
1651 Page Mill Road, Palo Alto, CA 94304; Rm 360 |
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Mike Leipold |
Mass Cytometry (CyTOF) Manager |
650-723-4984 |
mleipold at stanford.edu |
299 Campus Dr. Fairchild Science Bldg; Rm D033 |
| Xuhuai Ji | Genomics Manager | 650-723-5050 | xuhuai.ji at stanford.edu |
1651 Page Mill Road, Palo Alto, CA 94304; Rm 107 |
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Location |
Building |
Room |
Hours |
Location |
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Immunoassay & Cell Analysis Lab |
1651 Page Mill Road, |
360 |
9 AM - 5 PM |
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Genomics Lab |
1651 Page Mill Road, |
107 |
8 AM - 5 PM |
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Mass Cytometry & Flow |
299 Campus Dr. Fairchild Science Bldg |
D033 |
9 AM - 5 PM |
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As with all Stanford Service Centers, credit must be given to Human Immune Monitoring Center (HIMC) for data that results in a publication. If the work done at HIMC produces data resulting in a figure in a publication, you are required to acknowledge HIMC in the publication. Further, if HIMC staff members provided significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the PI), then it is expected that these individuals will be coauthors on the publication.
One of our CyTOFs was purchased with funds from a NIH S10 Shared Instrumentation Grant (S10RR027431-01). Please acknowledge this grant in your papers using CyTOF data.
| Name | Role | Phone | Location | |
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| Holden Maecker, PhD |
Director
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650.723.1671
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maecker@stanford.edu
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Fairchild Sci Bldg, Rm D039, 299 Campus Drive, Stanford, California 94305-5124
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| Debra Panasuk |
Finance Manager
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650.725.7483
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dpanasuk@stanford.edu
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Biomedical Innovations Building, 240 Pasteur Dr., Palo Alto, CA 94304-5422
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| Service Price List |
| ► Flow Cytometry (8) | |||
| Name | Description | Price | |
|---|---|---|---|
| CyTOF phenotyping | (view additional details) | Inquire | |
| CyTOF ICS (per stim) | (view additional details) | Inquire | |
| CyTOF phospho-flow (per stim) | (view additional details) | Inquire | |
| CyTOF instrument use (trained users) - per hour | Inquire | ||
| CyTOF instrument use+tech labor (assisted run) - per hour | Inquire | ||
| Flow phenotyping (Lyoplate) | (view additional details) | Inquire | |
| Phospho-flow | (view additional details) | Inquire | |
| Technical Consulting or Labor Time (Flow Cytometry) - per hour | Inquire | ||
| ► Immuno Assays (5) | |||
| Name | Description | Price | |
| Olink panel (per array) | Inquire | ||
| Luminex human 76-80-plex (includes 2 replicates per sample) | Inquire | ||
| Luminex mouse 39-48-plex (includes 2 replicates per sample) | Inquire | ||
| Custom Materials - Immuno Assays | (view additional details) | Inquire | |
| Technical Consulting or Labor Time (Immuno Assays) - per hour | Inquire | ||
| ► Molecular and Genomic Assays (21) | |||
| Name | Description | Price | |
| AbSeq for Rhapsody (per cartridge) | Inquire | ||
| BCR or TCR for Rhapsody (per cartridge) | Inquire | ||
| BD Rhapsody RNAseq (per cartridge) | Inquire | ||
| bulk BCRseq, custom primers (per sample) | Inquire | ||
| bulk TCRseq, custom primers (per sample) | Inquire | ||
| Sample Tags for BD Rhapsody RNAseq (per sample) | Inquire | ||
| SMARTer bulk BCRseq (per sample) | Inquire | ||
| SMARTer bulk TCRseq (per sample) | Inquire | ||
| Single-cell TCRseq (per 96-well plate) | Inquire | ||
| Fluidigm BioMark qPCR array (48.48) | Inquire | ||
| PAXgene RNA extraction | Inquire | ||
| DNAeasy extraction | Inquire | ||
| Trizol RNA or DNA | Inquire | ||
| cfRNA or cfDNA | Inquire | ||
| FFPE RNA | Inquire | ||
| Bioanalyzer DNA/RNA | Inquire | ||
| miRNA array (Agilent) | Inquire | ||
| Gene Array Data Analysis Service (Tech Labor) - per hour | Inquire | ||
| QIAseq Immune Gene Panel | Inquire | ||
| Custom Materials - Genomics | Inquire | ||
| Technical Consulting or Labor Time (Molecular and Genomic Assays) - per hour | Inquire | ||
| ► Other Services and Fees (3) | |||
| Name | Description | Price | |
| Custom Materials Charge | Inquire | ||
| Shipping from Stanford FedEx Charge | Inquire | ||
| Shipping to Stanford FedEx Charge | Inquire | ||
