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The Human Immune Monitoring Center (HIMC) is designed to be a comprehensive source for immune monitoring services for clinical and translational studies. We perform:
Immunoassays: Luminex and Olink Target-96 platforms Mass Cytometry (CyTOF): Immunophenotyping, Intracellular cytokines, Phospho-epitopes Flow Cytometry: Immunophenotyping, Intracellular cytokines, Phospho-epitopes Genomics: RNA/DNA extractions, Microfluidic qPCR arrays, single-cell RNAseq Goals of the HIMC include:
The HIMC was developed in 2007 by the Institute for Immunity, Transplantation, and Infection (Mark Davis, Ph.D., director) in conjunction with the Center for Clinical Immunology (C. Garrison Fathman, founder). Our Faculty Advisor is Mark Davis.
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All Non-Stanford Customers - Getting a Project Quote
Please register for an iLab account so that we can generate a quote for your project.
To get started with iLab: Go to http://sharedfacilities.stanford.edu to register for an iLab account. Click the blue sign-up button in the upper right corner. After you click submit, iLab Support will send you your login credentials.
Once logged in, click on 'list all cores' on the left side of your page. Locate the Human Immune Monitoring Center, then open the Request Services tab.
To set up your project and receive a quote for services: Request Services from the “External Users” > “initiate request” button.
There are two forms to complete…
Please be sure to click “save completed form”
2. Complete the HIMC Service Request Form to select from our menu of services.
Select services from the menus (enter the anticipated number of samples) then click “Add selected services” to save the selections to the form.
When done completing the form, click “submit completed form” (at the bottom of the page) to save the entire form.
Once the form is submitted, we will be able to see your project in iLab and can generate a quote for your request.
Confirming Your Project is a GO!
When you've agreed to the services listed and require no more edits from us, and you want to move forward with the work, please click the Researcher Agree button.
Please let us know if you have any questions.
Thank you, and we look forward to working with you!
Holden Maecker, PhD | Facility Director
Office (Rm D039, Fairchild Building): (650) 723-1671
Email: maecker at stanford.edu
Name |
Role |
Phone |
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Location |
Iris Herschmann |
Immunoassay Specialist |
650-723-4984 |
irish2@stanford.edu |
1651 Page Mill Road, Palo Alto, CA 94304; Rm 360 |
Mike Leipold |
Mass Cytometry (CyTOF) Manager |
650-723-4984 |
mleipold@stanford.edu |
299 Campus Dr. Fairchild Science Bldg; Rm D033 |
Xuhuai Ji | Genomics Manager | 650-723-5050 | xuhuai.ji@stanford.edu |
1651 Page Mill Road, Palo Alto, CA 94304; Rm 107 |
Location |
Building |
Room |
Hours |
Location |
Immunoassay & Cell Analysis Lab |
1651 Page Mill Road, |
360 |
9 AM - 5 PM |
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Genomics Lab |
1651 Page Mill Road, |
107 |
9 AM - 5 PM |
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Mass Cytometry & Flow |
299 Campus Dr. Fairchild Sci Bldg |
D033 |
9 AM - 5 PM |
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As with all Stanford Service Centers, credit must be given to Human Immune Monitoring Center (HIMC) for data that results in a publication. If the work done at HIMC produces data resulting in a figure in a publication, you are required to acknowledge HIMC in the publication. Further, if HIMC staff members provided significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the PI), then it is expected that these individuals will be coauthors on the publication.
One of our CyTOFs was purchased with funds from a NIH S10 Shared Instrumentation Grant (S10RR027431-01). Please acknowledge this grant in your papers using CyTOF data.
Name | Role | Phone | Location | |
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Holden Maecker, PhD |
Director
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650.723.1671
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maecker@stanford.edu
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Fairchild Sci Bldg, Rm D039, 299 Campus Drive, Stanford, California 94305-5124
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Debra Panasuk |
Finance Operations
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650.725.7483
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dpanasuk@stanford.edu
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Biomedical Innovations Building, 240 Pasteur Dr., Palo Alto, CA 94304-5422
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Service Price List |
► Flow Cytometry (10) | |||
Name | Description | Price | |
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CyTOF phenotyping | (view additional details) | Inquire | |
CyTOF ICS (per stim) | (view additional details) | Inquire | |
CyTOF phospho-flow (per stim) | (view additional details) | Inquire | |
CyTOF instrument use (trained users) - per hour | Inquire | ||
CyTOF instrument use+tech labor (assisted run) - per hour | Inquire | ||
MIBI standard imaging (per slide) | (view additional details) | Inquire | |
MIBI additional scan time (per hour) | (view additional details) | Inquire | |
Flow phenotyping (Lyoplate) | (view additional details) | Inquire | |
Phospho-flow | (view additional details) | Inquire | |
Tech labor-flow cytometry | Inquire | ||
► Immuno Assays (8) | |||
Name | Description | Price | |
Luminex mouse 64-plex (includes duplicate wells) | Inquire | ||
NULISAseq inflam panel (per plate) | Inquire | ||
Olink Target 48 panel (per plate) | Inquire | ||
Luminex human 115-plex (includes 2 replicates per sample) | Inquire | ||
Luminex human 48-plex (includes 2 replicates per sample) | Inquire | ||
Luminex mouse 39-48-plex (includes 2 replicates per sample) | Inquire | ||
Olink Target 96 panel (per plate) | Inquire | ||
Tech labor-immunoassays | Inquire | ||
► Molecular and Genomic Assays (16) | |||
Name | Description | Price | |
BD Rhapsody RNAseq (per cartridge) | Inquire | ||
AbSeq for Rhapsody (per cartridge) | Inquire | ||
BCR or TCR for Rhapsody (per cartridge) | Inquire | ||
Sample Tags for BD Rhapsody RNAseq (per sample) | Inquire | ||
SMARTer bulk TCRseq (per sample) | Inquire | ||
SMARTer bulk BCRseq (per sample) | Inquire | ||
Single-cell TCRseq (per 96-well plate) | Inquire | ||
Fluidigm BioMark qPCR array (48.48 or 2 x 12.12) | Inquire | ||
miRNA array (Agilent) | Inquire | ||
PAXgene RNA extraction | Inquire | ||
DNAeasy extraction | Inquire | ||
Trizol RNA or DNA | Inquire | ||
cfRNA or cfDNA | Inquire | ||
FFPE RNA | Inquire | ||
Bioanalyzer DNA/RNA | Inquire | ||
Tech Labor - gene array data analysis | Inquire | ||
► Other Services and Fees (1) | |||
Name | Description | Price | |
Custom Charge (Passthrough materials) | Inquire |