Cell Sciences Imaging Facility: Beckman Center

 

Overview of Services

 
 

 Welcome to the Cell Sciences Imaging Facility at the Beckman Center

 

The Cell Sciences Imaging Facility (CSIF) is a University service center that provides high resolution, state-of-the-art technologies for imaging and analyzing the molecular and structural organization of cells, tissue and bioengineered materials.  The CSIF operates two sites at Stanford University: The CSIF at Beckman Center and the CSIF at Shriram Center.  Both locations are open to all members of the Stanford community as well as to external academic and industry researchers (with approval).  You are on the CSIF's Beckman iLabs Solutions site.

The CSIF at Beckman houses both light and electron microscopy services. Light microscopy systems: Upright and inverted confocal laser scanning systems (Zeiss LSM880, AiryScan, AiryScan FAST; Leica SP8 with resonant), upright LEICA SP5 (with long working distance immersion lens) +FLIM and OMX SIM (STORM, TIRF) are accessible by users.  FESEM and TEM are available in our full service EM facility. Quick links to the microscopes scheduling calenders are below.    

The CSIF at Shriram provides access to and training on fluorescence and transmitted light microscopy including: laser scanning spectral confocal with AiryScan, multi-photon imaging (Zeiss LSM780, AiryScan, DeepSee), spinning disk confocal microscope with TIRF and photo-activation capabilities (Nikon SDC), FLIM and widefield (epi-fluorescence, Zeiss AxioImager) microscopes.  Click this link to access CSIF Shriram Center iLab site.

  

Fluorescence Microscopy Core (FMC)

The CSIF-FMC provides access to and training on high end fluorescent microscopes on an hourly fee basis. Current microscope technologies include super resolution (SIM, gSTED, PALM/STORM), laser scanning and spinning disk confocal, multi-photon, FLIM, TIRF and widefield deconvolution. 

Electron Microscopy Core (EMC)

The CSIF-EMC is a full service lab providing access to and training on transmission (TEM) and scanning (SEM) electron technologies as well as sample preparation, immuno-histochemitry staining, room temperature electron tomography, serial-section SEM, EM imaging and documentation.

Array Tomography Core (ATC)

The CSIF-ATC provides complete array tomography services and training, including sample prep, array generation and immunolocalization as well as access to automated fluorescence microscope and AT specific reconstruction software. 

Both the Beckman and the Shriram locations offer software for 3D volume rendering and image analysis (Bitplane Imaris, PerkinElmer Volocity, SVI Huygens Deconvolution, ImageJ FIJI, Becker&Hickl SPC Image). Training, as well as assistance with experimental design, is available on all key technologies.

The CSIF is a member of and is supported by:

The Beckman Center, Stanford Cancer Institute and BioX

 

Getting Started

  • Stanford Users:  LOGIN or REGISTER for an iLab account using valid Stanford credentials (SUNet ID).

 Leadership

Jon Mulholland | CSIF Director

Office (B050A):  (650) 725-7532

Lab    (B050B):  (650) 723-2449

Email:  jwm at stanford.edu

 

Staff

Name Role Phone (650) Email  Location
John Perrino EM Lab Manager - TEM   723-3462 jperrino at stanford.edu Beckman B001
Ibanri Phanwar Array Tomography Specialist 721-6989 ibanri.phanwar at stanford.edu Beckman B001
Ruth Yamawaki SEM Specialist - FESEM   yamawaki at stanford.edu Beckman B005
Jon Mulholland CSIF Director   725-7532 jwm at stanford.edu   Beckman B050A ,B051 Shriram B023
Kitty Lee Lab Services Manager - Confocals 723-2449  kamanl at stanford.edu Beckman B050B
Anum Khan Advanced Imaging Specialist - CODEX 725-1031 anumkhan at stanford.edu Beckman B005
Youngbin Lim Advanced Imaging Specialist - Lattice Light Sheet   yblim at stanford.edu Beckman B005
Cedric Espenel Shriram Manager  498-3559 espenel at stanford.edu Shriram B023

 

Location

Hours

Cell Sciences Imaging Facility

Stanford University Medical Center

Beckman Center B050, B005 (FMC)

Beckman Center B001, B005, B051 (EMC, ATC)

Stanford, CA 94305-5301

8 a.m. - 5 p.m., Monday to Friday  

24/7 access for trained users

 

 



Reservation Calendars Quick Links

    Publication Acknowledgement 

As with all Stanford Service Centers, credit must be given to Cell Sciences Imaging Facility for data that results in a publication. If the work done at Cell Sciences Imaging Facility produces data resulting in a figure in a publication, you are required to acknowledge Cell Sciences Imaging Facility in the publication. Further, if Cell Sciences Imaging Facility staff members provided significant experimental design, data interpretation, or other intellectual contribution (as evaluated by the PI), then it is expected that these individuals will be coauthors on the publication.

 

Additionally almost all of the CSIF's microscopes were obtained via NIH instrumentation grants and therefore require proper acknowmedgement. Please see the required NIH citation information included on the microscope's description page or contact the CSIF director.

 

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Contacts

Name Role Phone Email Location
Jon Mulholland
Director
 
650-725-7532
 
jwm at stanford.edu
 
B050A
 
John Perrino
EM Lab Manager
 
650-723-3462
 
jperrino at stanford.edu
 
B001
 
Kitty Lee
Lab Services Manager
 
650-723-2449
 
kamanl at stanford.edu
 
B050
 
Ibanri Phanwar
Array Tomography Specialist
 
650-721-6989
 
ibanri.phanwar at stanford.edu
 
B001
 
Ruth Yamawaki
SEM Specialist
 

 
yamawaki at stanford.edu
 
B005
 
Anum Khan
Advanced Microscopy Specialist
 
650-725-1031
 
anumkhan at stanford.edu
 
B005
 
Youngbin Lim
Lattice Light Sheet Specialist
 
650-724-8442
 
yblim at stanford.edu
 
B005
 

Instrumentation, Services, & Training Price List


Search available services: View: by category alphabetically
Array Tomography (ATC) Services (6)
EMC Equipment Fees (9)
EMC Services (20)
EMC Training (2)
Electron Microscopy Center (EMC) Microscopes (4)
FMC Microscopes (5)
FMC Services and Training (3)
FMC supplies (8)

Available Equipment and Resources

Array Tomography (ATC) Equipment (1)
Electron Microscope Core (EMC): Equipment (10)
Electron Microscope Core (EMC): Microscopes (4)
Fluorescence Microscope Core (FMC): Microscopes/Equipment (7)